消炎痛对人恶性黑素瘤Libr细胞系的生长抑制作用

Antifan inhibited cell growth in human melanoma libr cell line

目的:评价消炎痛对人恶性黑素瘤Libr细胞系的抑制作用。方法:应用不同剂量的消炎痛作用于人恶性黑素瘤Libr细胞系后,进行细胞形态观察,并用MTT法检测细胞生长情况和流式细胞仪FITCAnnexinV-PI染色法分析细胞凋亡情况。结果:Libr细胞在消炎痛作用下,细胞生长形态改变,电镜下可见典型凋亡细胞特征;细胞活力降低,呈药物剂量依赖性;流式细胞仪分析发现显著比例的凋亡细胞。结论:消炎痛能抑制人恶性黑素瘤Libr细胞系的生长,诱导细胞凋亡。

Objective To assess the inhibition of cell growth in human melanoma Libr cell line by antinfan. Methods Libr cells was exposed to antinfan at different concentration in vitro, and we observed cell morphology by phase contrast microscope and transmission electron microscope. The viability of cells was determined with MTT method, and the cell apoptosis analysis was conducted with flow cytometry. Results After antinfan treatment, Libr cells morphology changed and typital apoptosis appeared. Antinfan inhibited the growth of Libr cells in a dose-dependent manner. And cells showed significant apoptosis by flow cytometry analysis. Conclusion Antinfan could inhibit the cell growth and induce apoptosis in human melanoma Libr cell line.