顺铂诱导的人肺腺癌多药耐药细胞株辐射敏感性的体外研究

Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study

对顺铂(Cisplatin,CDDP)诱导的人肺腺癌多药耐药单克隆细胞株SPC-A-1／CDDP-4及其亲代 SPC-A-1细胞体外的辐射敏感性进行研究。分别在有氧和乏氧条件下检测SPC-A-1／CDDP-4细胞和 SPC-A-1细胞内谷胱甘肽(Glutathione,GSH)的含量,对SPC-A-1／CDDP-4细胞和SPC-A-1细胞体外的辐射敏感性进行研究,观察GSH生物合成抑制剂丁胱亚磺酰亚胺(Buthionine sulfoximine,BSO)对 SPC-A-1／CDDP-4细胞和SPC-A-1细胞的辐射增敏作用。实验结果表明:单克隆细胞株SPC-A-1／CDDP-4 不仅对化疗药:CDDP、阿霉素(Adriamycin,ADM)、甲氨蝶呤(Methotrexate,MTX)和长春新硷(Vincristine, VCR)具有多药耐药性,而且对137Csγ射线也具有一定的交叉耐受性;这可能与SPC-A-1/CDDP-4细胞的 GSH含量无论是在有氧或乏氧条件下,均高于亲代的SPC-A-1细胞有关;BSO在有氧和乏氧条件下对 SPC-A-1／CDDP-4细胞和SPC-A-1细胞均有辐射增敏作用,但是乏氧条件下的辐射增敏作用大于有氧,对 SPC-A-1／CDDP-14细胞的辐射增敏作用大于对SPC-A-1细胞的辐射增敏作用。

The study was to evaluate radiosensitivity of a monoclonal human lung adenocarcinoma cell line SPC-A-1/CDDP-4 with MDR phenotype induced by cisplatin （CDDP） compared with its parental cell SPC-A-1 in vitro. The glutathione （GSH） content and the radiosensitivity of SPC-A-1/CDDP-4 and SPC-A-1 cells were investigated in aerobic and under hypoxia, respectively. The radiosensitization effect of buthionine sulfoximine （BSO）, an inhibitor of ghitathione （GSH） synthesis, to SPC-A-1 /CDDP-4 and SPC-A-1 cells was observed. The results indicated that the monoclonal human lung adenocarcinoma cell line SPC-A-1/CDDP-4 showed, to some extent, a cross-resistance to ^137Cs γ-ray, in addition to its resistance to anticancer drugs （CDDP, ADM, MTX and VCR）. The GSH content of SPC-A-1 / CDDP-4 cells was higher than that of SPC-A-1 cells both in aerobic and under hypoxia which might account for it. BSO had radiosensitization effect to SPC-A-1/CDDP-4 and SPC-A-1 cells both in aerobic and under hypoxia, but it was stronger under hypoxia than in aerobic and it was stronger to SPC-A-1/ CDDP-4 cells than to SPC-A-1 cells.