组织工程活性真皮的构建研究

An experimental study on the preparation of artificial dermis

目的探讨用脱细胞真皮(ADM)、胶原海绵膜(SCM)、胶原凝胶构建活性真皮的可行性。方法从幼儿包皮中纯化、扩增成纤维细胞(Fb),将其分别种植于ADM、胶原凝胶、SCM上,观察细胞增殖及支架收缩等情况。结果Fb在ADM上不能成活;在SCM上增殖良好,人工真皮收缩不明显;在胶原凝胶中增殖活跃,但人工真皮收缩明显。结论利用SCM构建的人工真皮,是一种较理想的活性真皮。

Objective To build artificial dermis by using the acellular dermis, collagen membrane and collagen gel as scaffolds. Methods The fibroblasts were isolated from infant skin. The 3^rd generation cells were seeded into 3 different scaffolds. The artificial dermis was detected by HE staining, phase contrast microscope or scanning electron microscope. Results The fibroblasts implanted on the ADM began to rupture and died after 2 to 3 days. Though the fibroblasts proliferated well in collagen gel, the artificial dermis contracted obviously. Another artificial dermis contracted slightly by inoculating fibroblasts on collagen membrane, and the fibroblasts on them were in appropriate proliferation. Conclusion The artificial dermis built by collagen membrane as scaffolds has a preferable structure for an ideal substitute of skin.