T-2毒素对软骨细胞合成一氧化氮的影响

Effects of T-2 toxin on nitric oxide production in chondrocytes

目的探讨T-2毒素对软骨细胞合成一氧化氮(NO)的影响。方法胎儿软骨细胞体外培养,用MTT法检测软骨细胞存活率;用Griess重氮化法测定软骨细胞培养上清液中NO含量;用Western blot检测软骨细胞一氧化氮合酶(iNOS)的表达。结果T-2毒素在质量浓度在1-2 000μg/L的范围内,软骨细胞存活率对其呈较典型的浓度依赖关系和时间依赖关系,而且随着作用时间的延长,浓度依赖关系更为明显;T-2毒素刺激软骨细胞分泌NO和表达iNOS蛋白。结论T-2毒素引起的软骨细胞损伤和生长抑制与T-2毒素刺激软骨细胞表达iNOS蛋白和分泌NO增多有关。

Objective To investigate the effects of T-2 toxin on nitric oxide （NO） production and nitric oxide synthase （iNOS） expression in chondrocytes. Methods Human chondrocytes cultured in vitro were treated with different concentrations of T-2 toxin in different time （1--5 day）. Cell viability of the treated cells was measured by MTT assay; The levels of NO in culture media were detected by colorimetric method of Griess assay; iNOS protein was measured by Western blot. Results Dose-dependent and time dependent effects of T-2 toxin were found on T-2 toxin treated chondrocytes within a certain range of concentration （1- 2 000μg/L） and a periods of time （1-5 day）; The levels of NO in T-2 toxin treated culture media were higher than that of normal control group; Overexpression of iNOS was detected in T-2 toxin treated cells. Conclusion T-2 toxin can enhance NO production and up-regulate expression of iNOS. NO plays an important role in T-2 toxin induced growth inhibitory and toxic effects.