人参多糖对移植性人白血病模型小鼠作用观察

Effect of Ginseng polysaccharide on human leukemia SCID mouse model

目的建立重度联合免疫缺陷（SCID）小鼠人白血病模型,观察人参多糖在体内对白血病细胞的药理学作用.方法在SCID小鼠尾静脉分别接种K562细胞建立SCID小鼠人白血病模型,应用组织细胞化学、RT-PCR、流式细胞术、组织病理监测在给予人参多糖前后白血病模型SCID小鼠的外周血、骨髓、肝、脾中的白血病细胞.结果 SCID小鼠人白血病模型外周血象在疾病早期无明显变化,第18天白细胞数略有升高,光镜下可检测到1%～4%人白血病细胞,病程后期,白细胞明显增高,血涂片检查人白血病细胞占10%;经GPS注射后,SCID小鼠一般状况明显好于对照组,其肺、肾等脏器的炎症及病理变化减轻.结论人参多糖在体内能有效地治疗髓系白血病,且未发现有明显的毒副作用.

Objective To establish the human leukemia model in the severe combined immunodeficiency （SCID） mice and to investigate the pharmacological action of Ginseng polysaccharide （GPS） on leukemic cells in vivo. Methods The human leukemia mouse model was established by implanting K562 cells into the caudal vein of SCID mouse. Histochemistry, RT-PCR, flow cytometry, histopathology were used to monitor the leukemic cells in peripheral blood, bone marrow, liver, spleen of leukemia SCID mouse models, before and after injection of GPS. The mice receiving the physiological saline instead of GPS served as controls. Results The peripheral blood cells did not change so much in SCID mice in the earlier period of leukemia, the total number of WBC slightly increased and promyelocytes of leukemia could be detected 1% -4% on day 18 after K562 cells implantation. In last phase of leukemia, WBC significantly rose and could be monitored 10%. After injection of GPS, the common condition of mouse models was more improved than that of controls, the inflammation and pathological changes of organs ameliorated. Conclusion GPS can effectively suppress leukemia in vivo and is found no obvious ill effect.