大鼠神经干细胞缺氧后的内源性促红细胞生成素表达规律

Expression of endogenous erythropoietin of rat neural stem cells after hypoxia

[目的]观察神经干细胞缺氧后的内源性促红细胞生成素(erythropoietin,EPO)表达规律,初步探讨缺氧预处理诱导神经保护作用的机制。[方法]测定大鼠神经干细胞缺氧培养4 h后不同时间的内源性EPO表达情况,以及预处理后不同时间,再次遭受致死剂量缺氧伤害后的EPO表达情况。[结果]缺氧时间为4 h,EPO蛋白在处理后即刻出现表达,4 h达高峰,8 h仍有部分表达。再次遭受致死剂量缺氧伤害后,与预处理4 h组相比,预处理24 h组细胞的EPO表达量明显增多。[结论]缺氧预处理能够提高神经干细胞应对伤害刺激时内源性EPO的表达,并降低再次缺氧时诱导EPO表达的阈值。

[ Objective] To investigate the endogenous erythropoietin expression of neural stem cells（ NSCs ） of rats after hypoxia. [ Method] We used a rat NSCs culture model. EPO expression after 4 hours of hypoxia was studied, h was examined again following 12 hours of hypoxic lethal insult which was applied various times after hypoxic preconditioning. EPO expression was studied by western blotting. [ Result] Following the preconditioning, expression of EPO increased immediately and reached a peak 4 hours later. It lasted till 8 hours later. While applied the lethal hypoxia, EPO expression of cells in preconditioning 24 hours group were was significantly increased than that in preconditioning 4 hours group. [ Conclusion]The hypoxic preconditioning can lead an increased endogenous EPO expression and decrease the threshold of EPO expression against subsequent lethal stimulation during a certain period.