移植肾急性排斥时Fas配体和T细胞内抗原1的表达

Expression of Fas ligand and T cell intracellular antigen-1 during acute rejection after renal transplantation

目的:探讨移植肾发生急性排斥反应时肾组织Fas配体(Fas ligand,FasL)和T细胞内抗原1(T-cell intracellular an-tigen-1,TIA-1)的表达及意义。方法:应用免疫组织化学技术分别检测32例移植肾标本、2例供肾标本及8例肾癌周围肾组织标本FasL和TIA-1的表达,标本按Banff标准分为急性排斥(14例)、慢性排斥(15例)和无排斥改变(13例)3组,分析移植肾组织标本病理形态学改变与FasL和TIA-1表达的相关性。结果:FasL主要在肾小管上皮细胞胞质内表达,急性排斥组表达明显强于慢性排斥组和无排斥组(P<0.05);TIA-1在肾小管上皮细胞和间质浸润淋巴细胞均有表达,小动脉内皮细胞亦可见阳性着色,急性排斥组阳性强度较高,与慢性排斥组和无排斥组间存在显著差异(P<0.05);急性排斥标本FasL和TIA-1的表达强度与组织损害的严重程度呈正相关。结论:急性排斥时移植肾组织FasL和TIA-1表达增强是T细胞活化的反映,其检测可能有助于移植肾急性排斥的早期诊断。

Objective：To study the intrarenal expression of Fas ligand （FasL） and T cell intracellular antigen-1 （TIA-1） protein during acute rejection after renal transplantation. Methods： Immunohistochemical technique was used to detect FasL and TIA-1 expression in 32 transplanted renal samples,2 living donor kidneys and 8 adjacent samples of renal cancer （including 14 acute rejection samples, 15 chronic and 13 non-rejection ones according to Banff criteria）. Results： The positive FasL staining was observed mainly in cytoplama of renal tubule epithelia,and the positive rate was significantly higher in acute rejection samples than in others（P〈0.01 ）. TIA-1 was found in both tubular epithelial cells and interstitial infiltrating lymphocytes,occasionaly in small arterial endothelial cells. The positive staining rate of TIA-1 acute rejection samples was also higher than those of other samples（P〈0.05）. The expression of FasL and TIA-1 in acute rejection samples was positively correlated with the severity of tissue damages. Conclusion： Over expression of FasL and TIA-1 in acute rejection renal allograft tissues may indicate the activation of T cells,and its detection may be helpful for early diagnosis of acute rejection,