灯盏花素对异丙肾上腺素所致小鼠心肌肥大的保护作用

Effects of breviscapin on isoproterenol-induced myocardial hypertrophy in mice

目的:研究灯盏花素对异丙肾上腺素所致小鼠心肌肥大的保护作用。方法:以皮下注射异丙肾上腺素(3 mg.kg-1)诱发小鼠心肌肥大,小鼠灌胃灯盏花素药后30 min,给予异丙肾上腺素连续5 d。末次给药24 h后,处死小鼠,称取小鼠全心重量及左心室(含室间隔)重量,取左心室匀浆测定丙二醛(MDA)、超级氧化物歧化酶(SOD)、Ca2+-Mg2+-ATP酶活性和血清中天冬氨酸转氨酶(AST)。HE染色病理组织检查。结果:大剂量(60 mg.kg-1)灯盏花素和小剂量(30 mg.kg-1)灯盏花素均可降低心脏脏器指数,使心肌中MDA、AST水平降低,SOD、Ca2+-Mg2+-ATP酶活性升高(与对照组比较,P<0.05),病理检查心肌损害有明显改善。结论:灯盏花素可有效地预防异丙肾上腺素过量所导致的心肌肥大,与其抗氧化、改善心肌能量代谢有关。

AIM：To investigate effects of Breviscapin on isoproterenol- induced myocardial hypertrophy in mice. METHODS： Isoproterenol was given subcutaneously （3 mg.kg^-1, 5 days）to induce myocardial hypertrophy in mice. Therapy group were given Breviscapin 30 minutes before ISO treatment. We investigated the cardiac weight index and the malonyldialdehyde （MDA） content, the activity of superoxide dismutase（SOD）, Ca^2＋ -Mg^2＋ -ATPase in myocardium and aspartate aminotransferase （AST） in serum, Myocardiac morphological alteration was observed by micrography. RESULTS：In cardiac hypertrophy model group, HW/BW, LVW/BW （the ratio of whole heartweight to body weight and left ventricle weight to body weight）, and cardiac MDA, serum AST content were increased, the activity of SOD and Ca^2＋ -Mg^2＋ -ATP ase were decreased compared with control. Breviscapin improved all the above parameters significantly and improved the left ventricular tissue morphological alteration. CONCLUSION： Breviscapine possess protective effects against Isoproterenol induced myocardial hypertrophy in mice. This may be related to reducing the oxidative stress and improving energy metabolism.