UPLC-MS/MS联用法分析灯盏花乙素在胃肠道的代谢物

Study on metabolites of scutellarin in gastrointestinal tract by UPLC-MS/MS method

目的:研究灯盏花乙素在胃肠道的代谢物。方法:分别用盐酸、β-葡糖醛酸苷酶水解灯盏花乙素,并用健康志愿者的肠内菌对灯盏花乙素进行转化,用超高效液相-串联质谱联用法分析代谢物。结果:灯盏花乙素可被盐酸、β-葡糖醛酸苷酶水解为苷元,可被肠内菌群转化为苷元。健康受试者口服灯盏花乙素后,血浆中能检测到灯盏花乙素苷元。结论:健康受试者口服灯盏花乙素后,在吸收进入血液之前,胃肠道内灯盏花乙素与其苷元并存,而苷元更易于吸收,以总苷元为检测对象研究灯盏花乙素药代动力学是合理的。

AIM： The metabolites of scutellarin were studied in gastrointestinal tract. METHODS： Scutellarin was hydrolyzed with hydrochloric acid and β-glucuronidase. Human intestinal flora and scutellarin were incubated in vitro. The metabolites were identified by UPLC-MS/ MS method. RESULTS： Scutellarin could be hydrolyzed with hydrochloric acid and β-glucuronidase, and could be metabolized by human intestinal bacteria. A main metabolite, scutellarein, also was found in the plasma of healthy volunteers after oral administration. CONCLUSION： There were scutellarin and scutellarein in intestinal before absorption. It was suitable for scutellarin pharmacokinetic studies to determine scuteilarein or total aglycone.