白藜芦醇对离体犬冠状动脉血管环的影响

Effects of resveratrol on isolated coronary artery rings of canine

目的:研究白藜芦醇和17β-雌二醇对狗冠状动脉舒张特征的影响及其机制。方法:制备狗冠状动脉血管环,固定于盛有K-H液的恒温肌槽内,并观察等长时血管张力的变化。结果:白藜芦醇和17β-雌二醇使KCl及无钙K-H液中CaCl2量效曲线明显右移,使肌条敏感性和最大收缩反应明显降低。Nω-L-硝基精氨酸、甲烯兰、正矾酸钠及去细胞内皮,可部分阻断白藜芦醇和17β-雌二醇的舒张血管作用。格列本脲组对白藜芦醇和17β-雌二醇的舒张作用没有影响。结论:白藜芦醇和17β-雌二醇舒张血管都具有内皮依赖性,与KATP通道无关。

AIM： To investigate the relaxative characteristics of resveratrol （RES） and 17β-Estradiol （EST） on coronary arteries in dogs and its mechanism. METHODS： Rings of canine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution, and then isometric tension was measured. RESULTS： Both RES and EST caused the does-response contractile curve of KCl in K-H solution and CaCl2 in Ca^2＋ -free K-H solution shift rightward, and their sensitivies and the values of maximum contractions had been decreased. In the Nω-L-nitro-arginine, Methylene Blue and Sodium Orthovanadate groups, the vasorelaxant effects of both estrogens were markedly attenuated （P 〈 0.01 ）, but there was no change in the group of Glibenelamide （P 〉 0.05 ）. CONCLUSION： Resveratrol and 17β-Estradiol relaxe vascular smooth muscle in an endothelium-dependent manner, and are not related to KATP channel.