丹参治疗单纯疱疹病毒性脑炎小鼠脑损伤实验研究

Study on treatment of simple herpes encephalitis in mice with Salvia miltiorrhiza Bunge

目的:探讨丹参对单纯疱疹病毒I型(HerpesSimple Virus-1,HSV-Ⅰ)感染所致的小鼠病毒性脑炎的疗效及机理。方法:建立HSV-Ⅰ感染所致的病毒性脑损伤模型,观察小鼠的死亡率,检测小鼠血液中的细胞因子白介素-1β(IL-1β)、γ-干扰素(IFN-γ)、一氧化氮(NO)水平变化,以及电镜下小鼠神经细胞形态结构的变化。结果:电镜下HSV-I感染后小鼠脑细胞胞浆水肿明显,核仁固缩,核内结构破坏,多数线粒体呈空泡样改变,脊突破坏,髓鞘严重松解,核仁内可见病毒颗粒;IL-1βI、FN-γ、NO水平及小鼠的死亡率均明显上升。丹参治疗后小鼠脑组织病理改变减轻,死亡数及IL-1β、NO水平明显下降,而IFN-γ继续上升。结论:丹参早期应用对病毒性脑损伤有一定的疗效,对病毒性脑炎的临床用药有指导意义。其治疗病毒性脑损伤的作用机制可能是通过调节免疫应答,影响IL-1β、IFN-γ和NO等细胞因子的水平减轻炎症反应,改善脑损伤。

AIM： To explore the curative effect and mechanism of Salvia miltiorrhiza Bunge （SMB） against mice＇ s viral encephalitis induced by simple herpes virus-1 （HSV-1）. METHODS： To establish damage model of viral encephalitis in order to observe the mortality rate of mouse, to test the level change of the factors of cells such as IL-1β, IFN-γ, NO and to observe the structural change in neural ceils under the electron microscope. RESULTS： Cells swelled, nucleoli pyknosised and the structure of nucleus was damaged after been inoculated with HSV-1 under the electron microscope. There was hollow-like change in mitochondria and virus-like pellet in nucleus, spine was damaged, the levels of IL-1β, IFN-γ, NO and the death rate obviously increased in model group, and those indexes decreased after treating with SMB except IFN-γ. CONCLUSION： Early treated with SMB has certain clinical curative effect. It may result from regulating immune response in the body.