急性分离的肺泡Ⅱ型细胞的钠转运及调节

Sodium transport and its regulation of acutely isolated alveolar type Ⅱ cells

目的观察急性分离的肺泡Ⅱ性细胞全细胞钠电流及调节特性。方法应用膜片钳技术全细胞记录模式记录分离后5h内的大鼠ATⅡ的全细胞钠电流,并观察阿米洛利和特布他林对其的影响。结果可以记录到阿米洛利敏感性钠电流,应用特布他林后电流明显增大。结论可以利用急性分离的ATⅡ细胞进行钠通道电生理研究。为病理状态下的研究开拓了新的视野。

Objective To observe the sodium current of acutely isolated alveolar type Ⅱ cells and its regulation. Methods After isolation the AT Ⅱ cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect ofamiloride and terbutaline on the current was investigated. Results The amiloride-sensitive current of AT Ⅱ cells were recorded, which could be obviously stimulated by terbutaline. Conclusion Acutely isolated AT Ⅱ cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.