口蹄疫O-A-AsiaⅠ型三价灭活疫苗免疫效力和最小免疫剂量的研究

Studies on immune potency and minimum vaccinal dosage of trivalent inactivated vaccine against foot-and-mouth disease virus types O,A and AsiaⅠ

为确定口蹄疫O-A-AsiaⅠ型三价灭活疫苗的安全性和免疫效果，按OIE口蹄疫疫苗效检标准和我国口蹄疫灭活疫苗质量标准，对实验室条件下制备的4批疫苗进行了安全性试验、免疫效力试验、免疫持续期试验、疫苗保存期试验和最小免疫剂量试验。结果显示，4批疫苗均对靶动物和实验动物安全，无任何毒副反应；对O、A、AsiaⅠ型口蹄疫的平均免疫效力分别为4．73、6．84和8．10PD50／头份；一次接种的有效免疫期达6个月；2～8℃下的疫苗保存期为12个月；适宜的免疫剂量为，6月龄以上牛每头3．0mL，6月龄以下牛每头1．5mL。该疫苗对接种动物安全，免疫效果良好，一次接种可同时预防O、A、AsiaⅠ型3个血清型的口蹄疫。

In order to ascertain the safety and immune effect of the trivalent inactivated vaccine against foot-and-mouth disease virus types O, A and Asia Ⅰ , minimum vaccinal dosage, potency, immune duration, safety and storage life of the vaccine to target and experimental animals were detected with 4 batches of vaccines prepared under laboratory conditions according to the FMD vaccine standards of OIE and China. The 4 batches of vaccines had no any poisonous side effect to the target and experimental animals. The average potency to FMDV types O, A, and Asia Ⅰ were 4.73 PD50/dose, 6.84 PD50/dose and 8.10 PD50/dose, respectively. The immune duration could reach 6 months by one vaccination. The storage life of the vaccines was 12 months under 2-8 ℃. The suitable immunizing doses were 3.0 mL per cattle older than 6 months, and 1.5 mL per calf younger than 6 months. These results showed that the vaccines were high potent and safe to vaccinal animals and could prevent three types FMDV of O,A and Asia Ⅰ at the same time by one vaccination.