细胞外基质分子在人胚中肾有核血细胞的表达

Expression of extracellular matrix molecules in nucleared blood cells of mesonephros in human embryo

目的：观察细胞外基质分子在第4～8周人胚中肾有核血细胞的发育和迁移过程中的表达情况。方法：第4～8周的人胚行H—E和免疫组织化学染色，光镜观察。结果：发育第4、5周中肾的有核血细胞、原始肾小球、肾小管以及散在分布的间充质细胞均强表达纤连蛋白、层连蛋白、胶原蛋白Ⅰ、细胞间黏附分子。第7周，中肾有核血细胞中仅有部分表达上述分子。到第8周，仅在个别有核血细胞和肾小管上皮细胞表达。到第7周，中肾内有核血细胞几乎都表达整合素β1。肾内其他结构无明显的整合素β1表达。结论：在人胚发育第4～8周，中肾的有核血细胞表达多种细胞外基质分子。这些分子可能以自分泌方式参与肾内有核血细朐在中肾的发育。

Objective： To observe the expression of extracellular matrix molecules in nucleared blood cells in developmental mesonephros of human embryo of 4th- 8th week. Methods： Human embryo at 4th-8th week of gestation were collected with informed consent of woman suffered from accidental abortions. H-E staining and immunohistochemistry method （SABC） were employed. Results： All nucleared blood cells, glomerulus, mesonephric tubule and cells of mesoderm expressed fibronectin strongly on 4th week of gestation. Part of the nucleared blood cells expressed fibronectin, laminin, collagen Ⅰ and inter-cell adherin molecule; on the 7th week of gestation, the molecules were expressed in some nucleared blood cells and in a few cells on 8th week. All nucleared blood cells of mesonephros of 7th week expressed integrin β1. Conclusion： During 4th-8th week of gestation, nucleared blood cells express a series of extracellular matrix molecules, which may be involved in development of nucleared blood cells of mesonephros.