Fas/FasL、bcl-2、caspase-8在丹皮酚诱导大肠癌细胞凋亡中的相互作用及机制

The correlation of Fas/FasL, bcl-2, caspase-8 in paeonol inducing colorectal cancer cell line HT-29 apoptosis and molecule mechanism

目的探讨丹皮酚(Paeonol,Pae)诱导人大肠癌HT29细胞凋亡过程中Fas/FasL、bcl-2、caspase8细胞凋亡调节基因的相互关系及可能的作用机制。方法应用透射电镜技术对Pae诱导HT29细胞凋亡进行形态学观察;应用免疫细胞化学技术检测用药前后凋亡相关基因Fas/FasL、caspase-8、bcl-2表达的变化。结果HT29细胞经Pae作用后,在透射电镜下观察到HT29细胞出现典型的细胞凋亡形态;免疫细胞化学结果显示Pae作用后HT29细胞Fas/FasL、caspase-8蛋白表达明显升高,bcl2蛋白表达显著降低。结论Pae能抑制HT29细胞增殖并诱导其凋亡,其作用可能是通过上调Fas/FasL、caspase8蛋白表达,下调bcl2的表达有关。

Objective To investigate the correlation of Fas/FasL, bcl-2, caspase-8 in paeonol （Pae） inhibiting proliferation of HT-29 cells and inducing its apoptosis and the possible molecule mechanism. Methods The morphologiy of apoptosis on HT-29 cells were observed by transmission electron microscopy, The expression of apoptosis associated proteins Fas/ FasL,bcl-2,caspase-8 was measured by immunocytochemical staining. Results Typical apoptosis morphology of HT-29 cells was observed by transmission electron microscopy after treated by Pae, Immunocytochemical staining showed that the expression of Fas/FasL, caspase-8 proteins was increased significantly while the expression of bcl-2 protein was decreased on HT-29 cells after treated by Pae. Conclusion Pae may inhibit HT-29 cells proliferation and induce its apoptosis. The possible mechanism is up-regulation the expression of Fas/FasL, caspase-8 and down-regulation the expression of bcl-2 protein.