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esp1基因上调表达对A549细胞凋亡影响的研究 被引量：1

Effect of up-regulated esp1 gene expression on apoptosis of A549 cells
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摘要 目的观察A549细胞转染esp1基因后细胞凋亡情况。方法将含esp1基因的IREEGFP质粒用FuGENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系,检测转染细胞染色体数目及利用AnnexinV和TUNNEL试剂盒检测细胞凋亡的变化。结果A549细胞转入esp1基因后染色体异常分裂象减少,转染后细胞增殖减少,而去血清培养诱导的凋亡增加。结论esp1的上调表达不仅对于肿瘤的异常染色体分裂象以及肿瘤细胞的异常增殖有一定的抑制作用,且对A549细胞的凋亡有促进作用。 Objective To construct an expression vector containing esp1 in order to observe the effect of esp1 gene on the apoptosis of A549 ceils. Methods A conditional plasmid vector containing esp1 was constructed, and transfected into A549 ceils. The expression level of esp1 was detected by fluorescence microscopy, the chromosomes and the apoptosis, proliferation were detected. Results Transfection of IRE-EGFP-esp1 into A549 cells reduced improper chromosome segregation, cell proliferation and increased the apoptosis of A549 ceils. Conclusion Up-regulation of esp1 not only has inhibitory effects on the abnormal proliferation and chromatid separation of tumor ceils, but induces the apoptosis of A549 ceils.
作者 刘丽莎 刘昕
机构地区 第三军医大学基础医学部分子遗传学教研室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2006年第8期828-830,共3页 Journal of Third Military Medical University
关键词 A549细胞 esp1 转染 凋亡 A549 cell esp1 transfection apoptosis
分类号 R394-33 [医药卫生—医学遗传学] R394.2 [医药卫生—医学遗传学]
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参考文献11

  • 1CIOSK R,ZACHARIAE W,MICHAELIS C,et al.An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast[J].Cell,1998,93(3):1067-1076.
  • 2WAIZENEGGER I,GIMENEZ-ABIAN J F,WERNIC D,et al.Regulation of human separase by securin binding and autocleavage[J].Curr Biol,2002,12(16):1368-1378.
  • 3由莉越,刘昕.Separase上调对肿瘤细胞染色体分离作用的研究及条件表达载体的构建[J].第三军医大学学报,2005,27(6):497-499. 被引量：4
  • 4CHESTUKHIN A,PFEFFER C,MILLIGAN S,et al.Processing,localization,and requirement of human separase for normal anaphase progression[J].Proc Natl Acad Sci USA,2003,100(8):4574-4579.
  • 5NASMYTH K,PETER J M,UHLMANN F.Splitting the chromosome:cutting the ties that bind sister chromatids[J].Scince,2000,288(5470):1379-1385.
  • 6UHLMANN F.Secured cutting:Controlling separase at the metaphase to anaphase transition[J].EMBO Rep,2001,21(6):487-492.
  • 7HENGARTNER M O.The biochemistry of apoptosis[J].Nature,2000,407(6805):770-776.
  • 8UHLMANN F,WERNIC D,POUPART M A,et al.Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast[J].Cell,2000,103(3):375-386.
  • 9CHEN F,KAMRADT M,MULCAHY M,et al.Caspase proteolysis of the cohesion component RAD21 promotes apoptosis[J].J Biol Chem,2002,277(19):16775-16781.
  • 10PATI D,ZHANG N,PLON S E.Linking sister chromatid cohesion and apoptosis:role of RAD2[J].Mol Cell Biol,2002,22(23):8267-8277.

二级参考文献9

  • 1Ciosk R, Zachariae W, Michaelis C, et al. An ESp1/PDs1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast[J]. Cell, 1998, 93(6): 1067-1076.
  • 2Jensen S, Segal M, Clarke D J, et al. A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1[J]. J Cell Biol, 2001, 152(1): 27-40.
  • 3Uhlmann F, Wernic M, Poupart E, et al. Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast[J]. Cell, 2000, 103(3): 375-386.
  • 4Waizenegger I, Gimenez Abian J F, Wernic D, et al. Regulation of Human Separase by Securin Binding and Autocleavage[J]. Curr Biol, 2002, 12(16): 1368-1378.
  • 5Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters[J]. Proc Natl Acad Sci U S A, 1992, 89(12): 5547-5551.
  • 6Gossen M, Freundlieb S, Bender G, et al. Transcriptional activation by tetracyclines in mammalian cells[J]. Science, 1995, 268(5218): 1766-1769.
  • 7Gupta S, Schoer R A, Egan J E, et al. Inducible,reversible, and stable RNA interference in mammalian cells[J]. Proc Natl Acad Sci U S A, 2004, 101(7): 1927-1932.
  • 8Wiznerowicz M, Trono D. Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference[J]. J Virol, 2003, 77(16): 8957-8961.
  • 9萨姆布鲁克J 弗里奇EF 曼尼阿蒂斯T.分子克隆实验指南[M].北京：科学出版社,1999..

共引文献3

同被引文献10

  • 1刘丽莎,刘昕,刘永康.A549细胞转染esp1基因后生物学行为的变化[J].郑州大学学报（医学版）,2006,41(5):884-886. 被引量：1
  • 2刘丽莎,刘昕,程英.Tet-on系统诱导esp1基因表达对A_(549)细胞染色体的影响[J].重庆医学,2006,35(20):1862-1864. 被引量：1
  • 3Uhlmann F. The mechanism of sister chromatid cohesion[ J]. Exp Cell Res, 2004, 296( 1 ) :80 - 85.
  • 4Oueralt E, Uhlmann F. More than a separase [ J ]. Nat Cell Biol, 2005, 7 (10) : 930 -932.
  • 5Waizenegger I, Gimenez-Abian J F, Wernic D, et al. Regulation of human separase by securin binding and autocleavage [ J ]. Curr Biol, 2002, 12(16): 1368-1378.
  • 6Kumada K, Yao R, Kawaguchi T, et al. The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase[ J]. J Cell Biol,2006,172(6) :835 - 846.
  • 7Nagao K, Yanagida M. Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase[J]. Genes Cells, 2006, 11(3) : 247 -260,
  • 8Sullivan M, Hornig N C, Porstmann T, et al. Studies on substrate recognition by the budding yeast separase [ J ]. J Biol Chem, 2004, 279 (2) : 1191 -1196.
  • 9Shi Q, King R W. Chromosome nondisjunction yields tetraploid rather than aneuploid cells in human cell lines [J]. Nature, 2005, 437 (7061) : 1038 - 1042.
  • 10Potempa J, Sroka A, Imamura T, et al. Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of muhidomain protein complexes [J]. Curt Protein Pept Sci, 2003, 4(6) : 397 -407.

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第三军医大学学报
2006年 第8期

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