C-KIT蛋白在结直肠癌中的表达及临床意义

Expression and significance of C-KIT protein in human colorectal carcinoma

目的探讨CKIT蛋白在结直肠癌中的表达及其临床意义。方法应用免疫组织化学方法检测46例原发结直肠癌组织和30例癌旁正常组织的CKIT蛋白和CEA的表达。结果46例结直肠癌组织中,C-KIT蛋白表达率为15.2%(7/46),其中9例低分化结直肠癌组织中CKIT蛋白阳性表达率高达77.8%(7/9),而12例高分化、25例中分化结直肠癌组织及30例癌旁正常组织未见C-KIT蛋白表达;统计分析表明C-KIT蛋白的表达在不同肿瘤大小和临床分期的结直肠癌间无显著差异(P>0.05),而在不同癌细胞分化程度间差异显著(P<0.05)。结论表达C-KIT蛋白的结直肠癌细胞呈低分化趋向,提示以CKIT为靶点治疗低分化结直肠癌患者具有一定的可能性。

Objective To investigate the expression and significance of C-KIT protein in human colorectal carcinoma. Methods Expression of C-KIT was detected in 46 colorectal carcinoma specimens of different differentiation by immunohistochemistry, 30 normal colorectal specimens from paraneoplastic regions adjacent to colorectal carcinoma as control. Results Of the 46 carcinoma specimens, 7 out of 9 （77.8%） poorly differentiated colorectal carcinoma showed C-KIT positive staining in cytoplasm of cancer cells, but the expression of C-KIT was detected neither in the 12 well-differentiated and 25 moderately differentiated colorectal carcinomas nor in the 30 normal tissues, indicating that the expression of C-KIT may be related to differentiation of the cancer （P 〈 0.05）, not to the other characteristics of the Dukes＇ staging （P = 1. 000） and tumor size （P=0. 681 ）. Conclusion Expression of C-KIT protein may be associated with the poorly differentiated colorectal carcinoma. It suggested a possibility to treat poorly differentiated colorectal carcinomas by targeted interference in C-KIT signaling pathway.