兔干眼模型的建立

The Establishment of Rabbit Dry Eye Model

目的建立稳定的兔干眼模型,为进一步研究提供动物模型。方法新西兰大白兔14只,采用右眼摘除泪腺、第3眼睑及Harder's腺。造模后第1、3、5、7、10、15天行Sh irm erⅠ试验和1%虎红角膜染色检查,造模前与造模后术眼自身对照。造模后3周取角膜、结膜组织行光学显微镜及透射、扫描电镜检查眼表上皮细胞形态,左、右眼对照。结果造模前兔Sh irm erⅠ试验值(18.1±3.6)mm,造模后Sh irm erⅠ试验值随着时间的推移稳定下降,5 d后稳定在5.0 mm以下。造模后各天Sh irm erⅠ试验值与造模前比较均存在显著性差异(P<0.001)。造模前兔角膜虎红染色阴性,造模后7 d开始出现阳性染色,以后随着时间的推移染色程度逐渐加重。造模后3周术眼角膜、结膜光学显微镜及透射、扫描电镜检查均见异常。结论采用泪腺、第3眼睑和Harder's腺摘除术能成功建立兔干眼模型。

Objective To establish stable rabbit dry eye model for future research work. Methods Surgery was performed on 14 rabbits＇ right eye, by ablating the lacrimal gland, the tertiary eyelid and the Harder＇ s gland. Shirmer I test and 1% rose bengal staining were taken on 1,3,5,7, 10, 15 days after surgery. Enucleation was performed on 3 weeks after operation, and the excised cornea and conjunctiva were examined by light transmission and scanning electron microscopy. Results The values of Shirmer Ⅰ test before surgery was 18.1 ± 3.6 mm, but the values of Shirmer Ⅰ test after surgery decreased evidently and stably and all of the values were within 5 mm 5 days after surgery. The values of Shirrner I test showed significant difference before and 1,3,5,7,10,15 days after surgery. Before operation the result of 1% rose bengal staining were negative, but it became positive on 7 days after operation and the extent of staining was aggravated along with time. The examinations of cornea and conjunctiva by light transmission and scanning electron microscopy revealed abnormal. Conclusion Ablation of lacrimal gland, the teritiary eyelid and the Harder＇ s gland can establish rabbit dry eye model successfully and the method is simple and reliable.