缩胆囊素-A受体基因多态性与焦虑症的相关性

Association between Anxiety Disorder and Cholecystokinin-A Receptor Gene Polymorphism

目的探讨焦虑症(AD)与缩胆囊素-A(CCKA)受体基因多态性的相关性。方法运用PCR技术检测88例AD患者和87例健康对照(NC)组缩胆囊素-A受体基因多态性,并加以对照分析。结果CCKA受体基因的3种基因型(A1/A1、A1/A2和A2/A2),在AD组的分布分别为5%、41%和54%,在NC组分别为1%、44%和55%,两组间无显著性差异(P>0.05);A1和A2等位基因频率,在广泛性焦虑(GAD)组分别为34%、66%,惊恐障碍(PD)组分别为18%和82%,两组间均有显著性差异(P<0.05);A1/A1、A1/A2、A2/A2基因型分布频率,GAD组分别为5%、59%、36%,PD组分别为6%、24%和70%,两组间均有显著性差异(P<0.05)。结论CCKA受体基因多态性与AD之间无显著关联;AD两种亚型间CCKA受体基因多态性有显著性差异。

Objective To explore the association between anxiety disorder and cholecystokinin-A receptor （CCKAR） gene polymorphism. Methods Using the polymerase chain reaction-based technique, CCK-AR gene polymorphism was determined in 88 patients with anxiety disorder and 87 healthy controls. The frequencies of allelic and genotypic distributions between the two groups were compared. Results The frequencies of the three genotypes of CCK-AR receptor gene （A1/A1, A1/A2 and A2/A2） were 5% ,41% and 54% , respectively, in the patients with anxiety disorder and 1% ,44% and 55% , respectively, in the control（P 〉0.05）. There were significant differences in the frequencies of A1 and A2 allele genotype between patients with generalized anxiety disorder （34% and 66% ） and patients with panic disorder （18% and 82% ）. The frequencies of A1/A1 ,A1/A2 and A2/A2 genotype in patients with generalized anxiety disorder were 5% ,59% and 36% ,respectively ,and 6% ,24% and 70% ,respectively, in patients with panic disorder（P 〈 0.05）. Conclusion There was no significant association between CCK-AR gene polymorphism and anxiety disorder,while there were significant differences in CCK-AR gene polymorphism between the two subtypes of anxiety disorder.