体外垂体生长激素腺瘤转录靶向性基因治疗的实验研究

Transcriptional targeted gene therapy in vitro for growth hormone-secreting pituitary tumors

目的评价人生长激素启动子调控的基因治疗系统对垂体生长激素腺瘤的靶向性治疗作用。方法构建生长激素启动子调控的基因系统,体外转染垂体生长激素腺瘤GH3细胞;观察治疗基因HSV-TK在细胞中的表达靶向性,以及该系统杀伤细胞的能力和杀伤靶向性。结果HSV-TK蛋白能够在GH3细胞中靶向性表达,予以更昔洛韦后,GH3细胞增殖速度明显减慢,而对照细胞无明显杀伤作用(P<0.01)。结论生长激素启动子调控的基因治疗系统能有效地、靶向性地抑制GH3细胞增殖。

Objective To evaluate the targeting effect of human growth hormone promoter （hGHp） regulated gene therapy system on growth hormone-secreting pituitary tumors. Methods The hGHp regulated gene therapy system was constructed. GH3 cells （ growth hormone-secreting pituitary tumors cell lines） were treated with the gene therapy system. The targeting of the HSV-TK gene expression and gene therapy were observed. Results The HSV-TK protein only expressed in GH3 cells. The proliferating rate of GH3 cells treated by Ganciclovir was markedly lowered as compared with control cells （ P 〈 0. 01 ）. Conclusion The hGHp regulated gene therapy system can effectively and specifically inhibit the proliferation of GH3 cells.