高原急性低氧对大鼠食欲及下丘脑神经肽Y表达的影响

EFFECTS OF ACUTE HYPOXIA AT DIFFERENT SIMULATED HIGH ALTITUDE ON THE APPEPTITE AND EXPRESSION OF NEUROPEPTIDE Y IN HYPOTHALAMUS OF RATS

目的:探讨不同海拔高度急性低氧对大鼠食欲及其下丘脑神经肽Y(neuropeptideY,NPY)表达的影响。方法:Wistar大鼠随机分为平原对照组、3000、4000、5000和6000m低氧组,各低氧组缺氧时间分别为1、2、3d。观察低氧环境下大鼠食欲的变化;应用免疫组化法,研究大鼠下丘脑NPY的分布和表达。结果:(1)高原急性低氧后,大鼠食欲减退。(2)对照组和低氧组下丘脑NPY免疫阳性神经元在弓状核(ARC)、室旁核(PVN)和背中核(DMN)均有分布。低氧组NPY免疫阳性神经元数随海拔高度的上升和缺氧时间的延长逐渐减少。结论:高原急性低氧对大鼠食欲有抑制作用;急性低氧后下丘脑NPY表达降低,可能是大鼠食欲减退的重要原因之一。

Objective： To explore the effect of acute hypoxia on the appetite and expression of neuropeptide Y （NPY） in rat hypothalamus. Methods： Wistar rats were randomly divided into control group, 3 000 m group, 4 000 m group, 5 000 m group and 6 000 m group for different times （1d, 2d and 3d respectively）. The change of appetite was observed and the expression of NPY in hypothalamus of rats was measured by immunohistochemical method. Results： （1） After acute hypoxia, the appetite of rats decreased. （2） The expression of NPY of the test groups and the normal group was decreased in arcuatus, paraventricular and dorsal median nucleus of the hypothalamus after acute hypoxia. Conclusion： Acute hypoxia might inhibit feed intake of rats, and decreased expression of NPY in hypothalamus, which may be one of the important reasons for loss of appetite.