结肠癌患者血清IGF-1水平及癌组织VEGF表达的临床意义

Clinical Significance of Serum IGF-1 Level and Tissue VEGF Expression in Colon Cancer Patients

目的检测结肠癌患者血清中胰岛素样生长因子-1(IGF-1)的含量及癌组织中血管内皮生长因子(VEGF)的表达,并探讨其在结肠癌中的临床意义。方法应用双位点免疫放射测定法(IRMA)对不同Dukes分期的56例结肠癌患者术前术后血清IGF-1进行检测,应用SP免疫组化法检测各组结肠癌组织VEGF的表达,14例健康体检者设为正常对照。结果结肠癌患者术前血清IGF-1水平明显高于术后水平及正常对照,结肠癌组织VEGF表达率较正常对照组高,差异均有显著性意义(均P<0.05),不同病理分期的结肠癌血清IGF-1水平与癌组织VEGF阳性表达间差异有极显著性意义(P<0.01)。结论结肠癌患者血清IGF-1水平与结肠癌Dukes分期呈正相关,与癌组织VEGF表达密切相关,提示IGF-1和VEGF可能参与了结肠癌的发生发展过程,有望作为结肠癌诊治的重要检测指标。

Objective To detect the serum insulin-like growth factor-1 （IGF-1） level and tissue VEGF expression in pa tients with colon cancer and investigate its clinical significance. Methods The serum IGF-1 level was determined by immunoradiometric assay （IRMA） and the expression of tissue VEGF was detected by SP immunohistoehemical assay in 56 pre- and postoperative cases of colon cancer （based on Dukes staging）. Fourteen normal subjects were taken as control group. Results The serum IGF-1 level before operation was higher than that in control group and postoperation. VEGF expression rate was higher in tumor tissue than normal tissue, and the difference was significant （P〈0.05）. In patients with different pathological stages the serum IGF-1 level and tumor VEGF expression was statistically significant （P〈0. 01）. Conclusion The serum IGF-1 level and tumor VEGF expression in different stages of colon cancer was proportional to the severity of disease, so the serum IGF-1 level and VEGF expression may be an indicator of cancer occurrence and progression. Therefore, they may become an important diagnostic marker in colon cancer.