滤过速率对浓缩血小板去白细胞滤除效果实验评估

Experimental Evaluation on Effects of Leukodepletion Filtration of Platelet Concentrates at Different Speed Processes

目的 比较两种不同去白细胞过滤的速率对机采浓缩血小板（plateletapheresis concentrates，Pcs）质量的影响。方法 应用MCS＋机型、C5版本软件程序和采集管路中连接LRF系列血小板专用去白细胞滤器。PCs在采集过程中以5～８ml／min连续性低速过滤（L—LDP）或在采集结束后PＣs以25ml／min一次快速通过滤器（F—LDP）。PＣs产品质量预期分别为：血小板产量≥2．5×10^11胞数／袋和残余白细胞（WBC）数≤5．0×105／袋。结果 L—LDP和F—LDP方法获得的PＣs产品的血小板产量分别为（2．70±0．29）×10^11袋和（2．55±0．38）×10^11／袋（t=1．25），残余WBC数为（7．73±0．01）×10^4和（2．02±3．05）×10^5（10gt=1．48），采集成功率为78．6％和70．6％，以及采集效率为（57．1±6．8）％和（55．5±8．7）％（t=0．64）。L—LDP法获得PCs产品同时满足血小板产量和残余WBC数的双预期值的总体率占78．6％（22／28），而L—LDP仅为64．7％（11／17）。结论 两种去白细胞滤除方法都可以获得满意的效果。由于L—LDP的个性观察指标均好于F—LDP，提示慢速过滤获得的PCs产品质量更加稳定。

Objective To compare the quahty of platelet concentrates （PCs） between two different flow speeds of leukodepletion through filtration processes. Methods MCS ＋ version C5 and disposables were used, each of which linked with a leuko - depleted filter of LRF series products, The PCs was gone respectively through a continuous slow filtration process at flow rate about 5 to 8 ml per minute in collection process （L - LDP） and through a fast filtration process at flow rate about 25 ml per minute after collection （F- LDP） . The anticipation for PCs yield was ≥ 2.5 × 10^11 cells/bag and for residual white blood cells （WBC） was ≤ 5.0 × 10^5 cells/bag. Results The PCs products obtained by L- LDP and F- LDP correspondingly showed the mean platelet yield （2.70±0.29） × 10^11 cells/bag and （2.56±0.38） × 10^11 cells/bag （t = 1.25） the residual WBC （7.73 ± 0.01） × 10^4 cells/bag and （2.02 ± 3.05） × 10^5 cells/bag （log t = 1.48）, the success collection rate 78.6% and 70.6%, as well as the collection efficiency 57.1 ± 6.8% and 55.5 ＋ 8.7% （ t = 0.64） against precount. As both anticipated criteria of platelet yield and residual WBC level to be met synchronously, the PCs for L- LDP accounted for 78.6% （22/28） and the one for F - LDP hand only access to 64.7% （11/17） . Conclusion The satisfactory results for PCs were obtained through both different leukodepleted filtration processes. As all individualiged indexes observed of L - LDP excelled that of F - LDP, the PCs filtrated at low speed had a better quality than the one at fast speed.