膀胱尿路上皮癌突变型P53表达和微血管密度测定的意义

Relationship between the expressions mP53 and microvessel density in urothelial carcinoma of the bladder and its clinical pathological significance

目的:探讨膀胱尿路上皮癌(BUC)组织中突变型P53(mP53)表达和微血管密度(MVD)的关系及临床病理意义。方法:采用免疫组化二步法对50例BUC患者膀胱组织及10例正常膀胱组织进行mP53蛋白表达及MVD测定,观察两者之间的相关性,并与临床病理资料进行对比分析。结果:mP53的表达和MVD在正常人和BUC患者膀胱黏膜中差异非常显著(P<0.01);它们与BUC患者年龄、肿瘤多发无关,与BUC的病理分期、分级及复发显著相关(P<0.01);浸润型肿瘤组较表浅型肿瘤组mP53阳性表达率高(P<0.05),MVD也高(P<0.01);mP53阳性表达组及高MVD组复发率高(P<0.01)。结论:BUC组织中mP53的表达及MVD与BUC的病理分期、分级及复发显著相关,两者均为膀胱癌细胞生物学行为的标记物,对评估BUC的预后有重要意义。

Objective ：To investigate the relationship between the expression mP53 sity urothelial carcinoma of the bladder and its clinical pathological significance. and microvessel denhistochemistry（ Envision method） was used to detect the expression of mP53 and FⅧ-RAg in 50 tissue specimens of primary BUC and 10 tissue specimens of normal mucosa of bladder . The results were analyzed with the clinic and pathological characters of BUC. Results： The expression of mP53 and MVD had significant differences between the tissues of normal mucosa of the bladder and the tissues of the BUC ; but not related to the tumor size. mP53 and MVD value had correlation to different stages （ according to UICC-TNM ）, different grades （ pression rate of mP53 was higher in the according to WHO） , and recurrence（ P 〈0.01 ）. The positive exgroup of invasive stage（ P 〈 0.05 ）, and MVD was higher（P 〈 0.01）. The recurrent rate was higher in the group that the expression of mP53 was positive（ P 〈 0.01 ） and high MVD （ P 〈 0.01 ）. Conclusion ： The expression of mP53 and MVD have correlation to different stages , different grades and recurrent rate. The expression of mP53 and MVD have prognostic value in BUC.