高效液相色谱法快速测定满山红中槲皮素和杜鹃素的研究

Study on the Determination of Farrerol and Quercetin in Folium Rhododend Daurici by High Performance Liquid Chromatography

研究了用固相萃取预分离，高效液相色谱法测定满山红中槲皮素和杜鹃素。槲皮素和杜鹃素用90%甲醇加热回流提取，提取液用Waters Sep-Pak-C18固相萃取小柱预分离，然后用ZORBAX Stable Bound（50×4．6mm i．d．，1．8μm）色谱柱，以O．2％的磷酸和甲醇（52：48，V／v）为流动相，槲皮素和杜鹃素在1．0min内可达到基线分离；用紫外二极管矩阵检测器检测。方法的加入标准回收率为98％～102％，相对标准偏差为1．6％～1．8％。本法可用于满山红样品中的槲皮素和杜鹃素的测定，结果满意。

A high performance liquid chromatography （HPLC） method for the determination of farrerol and quercetin in Folium Rhododend Daurici was studied. Farrerol and quercetin were extracted from samples with 90% of methanol by heating circumfluence for 45 min. Then the extract was purified by solid phase extraction with Sep-Pak-C18 cartridge. Farrerol and quercetin were separated on a ZORBAX Stable Bound 50×4.6 mm i. d. , 1.8μm chromatographic column, with methanol and 0.20% phosphoric acid （52：48,V/V） as mobile phase at a flow-rate of 2.0 mL/min, and monitored with the photodiode array detector. The recoveries of farrerol and quereetin are 98%～102%, and relative standard deviations are 1.6%～1.8%. This method was applied to the determination of farrerol and quercetin in Folium Rhododend Daurici with good results.