均匀设计法优选白花败酱总皂苷的提取工艺

Extraction of total-saponins from Patrinia Villosa Juss based on uniform design method

目的:确定白花败酱总皂苷的最佳提取工艺。方法:采用均匀设计实验优选白花败酱中总皂苷的提取条件,用AB-8大孔吸附树脂柱对提取物进行提纯,并以齐墩果酸标准品作对照,采用薄层比色法进行含量测定。结果:确定最佳提取工艺为:乙醇浓度95%、溶剂用量8倍(5倍,3倍)、提取时间1 h(0.75 h,0.25 h)提取两次,实际白花败酱中总皂苷(以齐墩果酸计)含量为0.668%,与理论推测相符。结论:最佳提取工艺和提纯方法可以有效提取白花败酱中的总皂苷,工艺操作简单易行,适合工业生产。

AIM： To confirm the optimum extraction of the total-saponins from Patrinia Villosa Juss. METHODS： Optimum conditions （ethanol concentration, extraction hours, ethanol solution quantity） were determined by the uniform design test, the extract was refined with macropore resin AB-8, and samples were measured colorimet- rically at λ = 560 nm compared with oleanolic acid as reference substance. RESULTS： The result was 95% ethanol as solvent, extraction time for 1 h adding 8 times amount of ethanol solution at 100℃ water bath for two times. under this condition, the total-saponins content was 0.668%, in accordance with the design forecast. CONCLUSION： The optimum extraction technique and purified methods could extract the total-saponins efficiently from Patrinia Villosa Juss. The technique is simple and adapts for production.