毒性中药狼毒质量标准研究

Quality standard for Euphorbia

目的：建立毒性中药狼毒的质量标准。方法：采用硅微、化学、液相色谱／质谱等方法进行定性鉴别，以液相色谱及液相／质谱法测定含量。结果：狼毒大戟中月腺大戟乙素含量为0．01％w／w，月腺大戟中月腺大戟乙素含量范围为0．01％-0．02％w／w，均末枪出瑞香内酯、狼毒色原酮和新狼毒素A。瑞香狼毒中瑞香内酯、狼毒色原酮及新狼毒素A等成分含量范围分别为0．57％～2．12％w／w、0．86％-1．6％w／w及0．45％-0．96％w／w，但未检出含月腺大戟乙素成分。结论：各法能用于常规榆测狼毒属植物。

AIM ： Euphorbia is the unprocessed root of Euphorbia ebracteolata Hayata or Euphorbia Fischeriana Steud, or Stellera chamaejasme L. To establish the quality standard for these potent herbs. METHODS： The microscope examination techniques, physio-chemical methods such as HPLC/UV and LC-MS-MS had been used. RESULTS： The content of ebracteolata compund B in E. Fischeriana was 0.01%,and in E. ebracteolata was 0.01% -0.02%. But the skimmetine, chamaechromone and neochamaejasmin A had not been chekout in both species. The content of skimmetine, chamaechromone and neochamaejasmin A was 0.57% -2.12 %, 0.86% - 1.6%, and 0.45% -0.96% in S. chamaejasme. But the ebracteolata compund B had not been chekout in this specie. CONCLUSION： The method can be applied to conventional testing of Euphorbia.