生理条件下对苯二酚的电化学行为及电极产物的色谱鉴定

ELECTROCHEMICAL BEHAVIOR OF HYDROQUINONE UNDER PHYSIOLOGICAL CONDITIONS AND HPLC IDENTIFICATION OF ITS ELECTRODE REACTION PRODUCTS

用循环伏安法研究了对苯二酚在玻碳电极上的电化学行为，从循环伏安图上看出当在0至1．6V电位间连续扫描20次，在0．63V左右可见到一氧化峰电位，其峰电流随扫描次数的增加而减小。说明电极反应为不可逆过程。然而，如改变在-1．4～1.6V电位间扫描，仅数次扫描后即可在Epa=0．25V和Epc=-0．08V处见到一对氧化还原峰电位，说明了此过程为一可逆过程。同时，对电极反应的产物用RHPLC进行研究和鉴定，从色谱图所示结果与电化学法所得结果一致。根据此工作的研究结果对苯二酚在呼吸链中所产生的作用进行了探讨。

The electrochemical behavior of hydroquinone at glassy carbon electrode was studied by cyclic voltammetry, and it was found from the cyclic voltammogram that when scanning successively for 20 times in the potential range of 0 to 1.6 V, an oxidation peak potential （Epa） was observed at around 0. 63 V, and its peak current was decreased with the increase of time of scanning, showing that the electrode reaction was an irreversible process, and that when the potential range was change to -1.4-1. 6 V and scanned successively only for several times, a pair of redox peak potentials was observed at Epa=0. 25 V and Epc =-0. 08 V, showing a reversible process at the electrode. The reaction products at the electrode were studied and identified by RHPLC, the results obtained as shown by the chromatograms were in consistency with those of electrochemical method. Based on the results obtained, the important role of hydroquinone in the respiratory chain was discussed.