三氯乙烯对裸鼠皮肤的刺激性损伤及维生素E的保护作用

Damaging effect of trichloroethylene on skin and the protective effect of vitamine E

目的研究有机溶剂三氯乙烯(TCE)对BALB/c裸鼠皮肤组织的氧化损伤以及维生素E的保护作用。方法将BALB/c裸鼠随机分成100%TCE、80%TCE、40%TCE、20%TCE、10%TCE、溶剂对照组(橄榄油)、阳性对照组(5%SLS)、空白组和5%、10%、20%维生素E保护组,背部皮肤染毒1 d,观察皮肤损伤反应,取染毒皮肤进行实验,比较各组MDA含量、SOD活性。结果各染毒剂量组皮肤组织的MDA含量、SOD活性的差异有统计学意义,TCE浓度越高,氧化应激水平越高,但维生素E保护组氧化应激水平不高。结论TCE可通过氧化应激对皮肤组织造成损伤,维生素E在一定浓度下对皮肤组织有保护作用。

Objective To study the damaging effect of trichloroethylene, a organic solvent, on skin and the protective effect of vitamin E on it. Method BALB/c mice were randomly divided into several groups which were exposed to different concentrations of trichloroethylene, such as 100%TCE group, 80%TCE group, dO%TCE group, 20%TCE group, 10%TCE group, solvent （olive oil） control group, positive control （SLS） group, 5% VitE treated group, 10% VitE treated group, 20% VitE treated group and control group. The skin on the back of BALB/c mice were exposed to TCE for one day, observing the damage of the skins and detecting the level of MDA and activity of SOD in skin with kits. Result The MDA contents in TCE-exposed groups were gradually rose with the concentrations of TCE, the SOD activities were dropped ; while the MDA levels and SOD activities in vitamine E treated groups were gradually declined and rose respectively with the increase of Vit E concentration. Conclusion Trichloroethylene may induce skin damage through its oxidation stress effect and vitamin E shows some protective effect on skin because of its antioxidative effect.