三氯异氰尿酸对大鼠的亚慢性毒性

The subchronic toxicity of trichloroisocyanuric acid (TCICA) on rats

将SD大鼠按性别、体重随机分为高剂量组(1/8LD50)、中剂量组(1/16 LD50)、低剂量组(1/64LD50)和阴性对照组,分别喂饲相应的受试物饲料90 d,观察大鼠体重、血常规、尿常规、血清生化、脏体系数和组织病理学变化。结果与对照组相比,高剂量组雌性大鼠在染毒第70 d后生长明显减缓并持续到实验结束,AST、ALT活性明显升高;雄性大鼠睾丸脏体系数明显增大,AST、BUN显著升高。中剂量组雄性大鼠AST、BUN也明显增高。低剂量组各指标变化不明显。各剂量组脏器组织未见明显异常改变。提示三氯异氰尿酸原药对动物体重增长有抑制作用,对肝、肾功能有不良影响。经口染毒的亚慢性毒性阈剂量雌雄大鼠分别为99.25、49.62 mg/kg,最大无作用剂量雌雄大鼠分别为49.62、12.41 mg/kg。

The SD rats were randomly divided into high dosage group （1/8LD50）, middle dosage group （1/16LD50）, low dosage group （1/64LD50） and control group accorchng sex and body weight. After 90-day feeding with the feeds contained various contents of TCICA, the examinations on body weight, blood and urine routines, blood biochemistry indices, organ relative weight and pathological changes were performed. Result showed that comparing with the controls, the female rats in high dosage group showed slow growth from 70th day after exposure to TCICA and kept until the end of the experiment, the serum activeties of AST and ALT increased obviously; the male rats showed obvious accretion in the ratio of testis weight/body weight, and rise in serum AST activity and BUN level. The serum AST activity and BUN level in middle dosage group were also increased; whlie in low dosage group, no obvious change could be seen, and no any pathological change occurred in all the experimental rats, Trichlomisocyanuric acid has the inhibition effect on growth of body weight, and also has some harmful effects on the function of liver and kidney. The subehronic oral threshold dosages of TCICA for female and male rats are 99.25 mg/kg and 49.62 mg/kg respectively, and its maximal non-effective dosage for female and male rats are 49.62 mg/kg and 12.41 mg/kg respectively.