硒协同茯苓多糖抗小鼠白血病的实验研究

Experimental study on antileukemia effect of pachymaran in combination with selenium

目的研究硒协同茯苓多糖(CMP)对P388小鼠白血病的抗癌效果并探讨其治疗的分子机制。方法硒协同CMP治疗P388白血病模型,通过观察荷瘤小鼠生存期判断抑癌效果。采用原位杂交和免疫细胞化学技术检测淋巴细胞bcl-2基因;利用流式细胞术检测细胞凋亡、增生指数(PI)及细胞周期。结果CMP、硒与环磷酰胺联合用药使荷瘤小鼠生命延长75.09%;PI降低;bcl-2基因表达下调,凋亡指数增高,但凋亡率比单独环磷酰胺组低。结论CMP、硒与化疗药物合用后具有协同抗癌效应,能显著抑制癌细胞增生,下调bcl-2基因的表达、诱导癌细胞凋亡,从而延长荷瘤小鼠的生存期,同时减轻环磷酰胺的毒副作用。

Objective To study the antileukemia effect of pachymaran in combination with selenium on P388 leukemia mice and its molecular mechanism of antileukemia. Methods The P388 murine leukemia model was administrated with pachymaran in combination with selenium. In situ hybridization （ISH） and immunocytochemistry assay were used for the expression of bcl-2 gene. Flow cytometry was used to assess the extent of cell apoptosis, proliferation index and cell cycle .The effect of antileukemia was determined by observing the life span of P388 bearing mice. Results The life spans of combination of pachymaran and selenium and cyclophosphamide （CTX） group prolonged survival 75.09 % and downregulated markedly the expression of bcl-2 gene to raise apoptosis index and prevented the inhibition of cancer cell, but the rate of apoptosis was lower than CTX group. Conclusions Combination of pachymaran and selenium and CTX group enhanced markedly the antileukemia effect and downregulated the expression of bc1-2 gene and induced apoptosis to make life spans of P388-bearing mice long and reduce the toxicant of CTX.