消化病患者胃肠引流液中幽门螺杆菌检出率和患者VitC水平(英文)

Helicobacter pylori infection in gastrointestinal drainage juice and of vitamin C levels in patients with digestive diseases

目的探讨胃肠引流液中幽门螺杆菌(Helicobacterpylori,H.pylori)的检出率及患者VitC水平。方法用PCR技术、细菌培养、快速尿素酶试验检测胃肠引流液中的H.pylori,用反相液相色谱法检测患者胃肠引流液及血浆中VitC含量。结果282例患者胃肠引流液中H.pylori检出率为41.1%(116/282),PCR、细菌培养及快速尿素酶试验阳性率分别为42.9%(121/282)、11.3%(32/282)、41.1%(116/282)。胃溃疡、十二指肠溃疡、复合溃疡、胃癌、急性肠梗阻、急性胆囊炎及门静脉高压合并食管胃底静脉曲张患者H.pylori检出率分别为54.6%(30/55)、52.2%(24/46)、58.3%(7/12)、48.2%(27/56)、22.2%(8/36)、27.6%(16/58)、21.1%(4/19),差异具显著性(P<0.01)。胃溃疡、十二指肠溃疡、复合溃疡、胃癌H.pylori阳性患者与H.pylori阴性患者血浆及胃肠引流液VitC浓度相比,差异均具有显著性(P<0.01)。结论胃肠引流液中可检出H.pylori,且能分离培养出活菌;PCR技术是一种敏感、有效的检测胃引流液中H.pylori的方法。H.pylori感染人体后引起胃肠引流液及血浆中VitC含量的减少,可能与胃十二指肠疾病的发生有关。

Objective To investigate the infection situation of H.pylori in gastrointestinal drainage juice and detect levels of vitamin C in patients with digestive diseases. Methods H.pylori in gastrointestinal drainage juice was detected with PCR （polymerase chain reaction）, bacteria cultivation and rapid urease test, and the vitamin C levels in gastrointestinal drainage juice and plasma of 282 patients with gastroenteropathy was detected with reverse liquid chromatography. Results The infection rate of H.pylori in gastrointestinal drainage juice was 41.1% （116/282）, the positive rates of H.pylori with the methods of PCR, bacteria cultivation and rapid urease test were 42.9%, 11.3% and 41.1%, respectively. The infection rates of H.pylori in patients with gastric ulcer, duodenal ulcer, mixed ulcer, gastric cancer, acute ileus, acute cholecystitis and portal hypertension with varicosis were 54.6% （30/55）, 52.2% （24/46）, 58.3% （7/12）, 48.2% （27/56）, 22.2% （8/36）, 27.6% （16/ 58） and 21.1% （4/19） respectively, with a statistically significant difference among them （P〈0.01）. In addition, there was a significant difference in vitamin C levels between H.pylori-positive and negative patients with gastric ulcer, duodenal ulcer, mixed ulcer and gastric cancer （P〈0.01）. Conclusion H.pylori exist in gastrointestinal drainage juice. PCR is a sensitive and convenient method for detection of H.pylori in gastrointestinal drainage juice. The vitamin C levels in gastrointestinal drainage juice and plasma decrease in patients with H.pylori infection, which may be associated with the occurrence of gastric and duodenal diseases.