图象分析仪DNA分析在痰液细胞学诊断中的应用

The cytological diagnosis value of DNA analysis by ICM in sputum examination

目的 评价DNA分析在痰液涂片细胞学检查中作用和意义。方法 对HE染色痰涂片褪色后行Feulgen染色，用HPIAS-1000型高清晰度彩色全自动分析系统作DNA分析。结果 35例肺癌细胞及9例不典型支气管上皮细胞DNA均为异倍体（heteroploid，H）核型。两组细胞DNA二倍体，异倍体（2C／H）核型分别占37．1％和66．6％（P〈0．001），五倍体/非整倍体（5C／AN）核型分别占71．4％、0．0％（P〈0．001），非整倍体（AN）核型占97．1％、0．0％（P〈0．001）；而增生期百分比（PF）分别占（83．8±19．7）％、（47．629±．5）％（P〈0．001）。癌细胞组29例非小细胞癌与6例小细胞癌的2C／H核型分别占44．8％和0．0％（P〈0．001），5C／AN占65．5％、100．0％（P〈0．001），非整倍体（AN）核型为96．5％、100．0％（P〉0．05）；而PF值为（83．1±20．1）％、（95．0±2．0）％（P〈0．02）。结论 痰液细胞DNA分析指标（倍体核型、增殖期百分比）可作为细胞学肺癌诊断的重要理论参考。

AIM To evaluate diagnostic role and significance of DNA analysis in sputum examination. Methods Feulgen staining performed in destained HE staining, and measured DNA content with HPIAS-1000 image cytometry （ICM）. Results In 35 cases with lung carcinoma and 9 cases with atypical bronchus epithelium, The positive rate of heteroploid （H） were both 100.0%, 2C/H were 37.1%, 66.6% respectively （P〈0.001）, 5C/AN were 71.4%, 0.0% respectively （P〈0.001）, aneuploid （AN） were 97.1%, 0.0% respectively （P〈0.001）; the percentage of proliferous phase fraction （PF） were （83.8± 19.7）%, （47.6 ±29.5）% respectively （P〈0.001）. in 29 cases with non-small-cell carcinoma and 6 cases with small cell carcinoma, The positive rate of 2C/H were 44.8%, 0.0% respectively（P〈0.001）, 5C/AN were 65.5%, 100.0% respectively （P〈0.001）, aneuploid （AN）were 96.5%, 100.0% respectively （P〉0.05） ; PF were （83.1±20.1）%, （95.0±2.0）% respectively （P〈0.02）. Conclusion DNA analysis was used as an important reference for cytology diagnosis of lung carcinoma.