等度高效液相色谱测定大豆异黄酮中大豆甙元和金雀异黄酮

Determination of the Daidzein and the Genistein in the Soy Bean Isoflanoves by Isocratic Elution High Performance Liquid Chromatography

本文介绍了等度高效液相色谱(HPLC)测定大豆异黄酮中二种主要成份:大豆甙元(Daidzein)和金雀异黄酮(Genistein)的方法。采用Atlantis C18色谱柱和EasyGuard C18,保护柱;以甲醇:0·1%醋酸(pH3·11)=51·5:48·5(v/v)为流动相;检测波长λ=254nm;流速1mL/min。在测定范围内(10-200ng)峰面积与质量浓度线性关系良好。大豆甙元和金雀异黄酮的相关系数分别为0·9984,0·9997·两组份回收率为97·0—102·92%·

An analytical approach has been developed for isocratic elution high performance liquid chromatographic determination of Daidzein and Genistein in the soy bean isoflanoves. They were measured using Atlantis C18 for analytical column and EasyGuard column C18 for guard column,and UV detector（at 254nm）. The mobile phase was methanol ： 0. 1% acetic acid （pH3.11） = 51.5：48. 5 （v/v）. there was a good linear relationship between the content of Daidzein, Genistein and its peak area, with the correlation coefficients of 0. 9984 and 0. 9997 respectively, Average recoveries were 97.0% - 103.0%.