摘要
参照PRRSV ATCC VR2332株基因序列设计1对引物P1、P2,经RT-PCR扩增出PRRSV SD-1株ORF7基因,经与PMD18-T Vector连接后筛选出阳性克隆株测序。把PRRSV SD-1株ORF7 cDNA亚克隆到PQE上构建PQE-N原核重组表达载体。结果表明,PRRSV SD-1株ORF7核甘酸序列与PRRSV ATCC VR2332株ORF7核甘酸序列完全一致,与欧洲株LV符合率为58%。成功构建原核表达载体PQE-N,为N蛋白的研究和制备诊断性抗原奠定了基础。
ORF7 gene of porcine reproduetive and respiratory syndrome virus（PRRSV） SD-1 was amplified by RT-PCR and ligated to PMD18-T, sequencing the ORF7 of PRRSV SD-1 after seeking out the recombination plasmid. The primers were designed according to the sequence of PRRSV ATCC VR2332. The PQEN prokaryotic expression vector was constructed by subclone the eDNA of ORF7 into PQE. The results indicated that the homologus nucleotide sequence of ORF7 of PRRSV SD-1 is 100% and 58% compared with PRRSV ATCC VR2332 and LV,respectively. In conclusion,PQE-N was constructed, it was a base to the research of N protein and production of diagnosis antigen.
出处
《动物医学进展》
CSCD
2006年第4期64-68,共5页
Progress In Veterinary Medicine
基金
山东省自然基金重大项目(Z2000D03)
