人参对老年大鼠脑组织β肾上腺素能受体含量的影响

Effect of ginseng on beta-adrenergic receptor content in brain tissues of aged rats

目的:观察具有大补元气、生津止渴、安神益智功效的人参对老年大鼠不同脑区β肾上腺素能受体含量的影响,分析该作用是否有剂量依赖性。方法:实验于2003-05/2004-12在中山大学中山医学院人体解剖学教研室的脑研究室完成。①选用清洁级健康SD大鼠30只,雌雄不拘,体质量400～600g,18～20月龄。按随机数字表法将大鼠分为3组:对照组、高剂量人参水煎液组、低剂量人参水煎液组,每组10只。②高剂量人参水煎液组:灌胃含生药1.5g/(kg·d)人参水煎液(吉林生晒参,均购于广州药材公司。分别第1次用8倍水分煎2h,第2次6倍水分煎1.5h,浓缩至含生药2kg/L)3mL;低剂量人参水煎液组:灌胃含生药0.5g/(kg·d)人参水煎液3mL;对照组:灌胃蒸馏水3mL,连续3周。③3周后,麻醉大鼠,开颅分离前额皮质、海马、小脑皮质、丘脑、下丘脑、脑桥、脊髓颈段。用放射配基受体结合分析法测定各组大鼠前额皮质、海马、小脑皮质、丘脑、下丘脑、脑桥、脊髓颈段β肾上腺素能受体含量。④先行单因素方差分析,再用Student-Newman-keals方法对各组数据进行统计学处理。结果:老年大鼠30只均进入结果分析。①中枢皮质结构各脑区肾上腺素能受体含量:给药3周后,高、低剂量人参水煎剂组大鼠前额皮质、海马、小脑皮质的β肾上腺素能受体含量均明显高于对照组(t=2.71～4.21,P<0.05～0.01),其中高剂量人参水煎剂组作用更为明显。②中枢皮质下结构各脑区β肾上腺素能受体含量:给药3周后各组大鼠丘脑、下丘脑、脑桥、脊髓颈段β肾上腺素能受体含量比较,差异不明显(P>0.05)。结论:人参可选择性提高老年鼠前额皮质、海马、小脑皮质β肾上腺素能受体含量,且高剂量人参水煎液[含生药1.5g/(kg·d)]作用更为明显,但对丘脑、下丘脑、脑桥、脊髓颈段β肾上腺素能受体含量无明显影响。

AIM： To observe the effect of ginseng on beta-adrenergic receptor （β-AR, invigorating primordial qi potently, promoting production of body fluid to quench thirst and tranquilization） contents indifferent brain tissues of aged rats, and analyze the dosage-dependence of this effect. METHODS： The-experiment was carried out at the Brain Institute,Department of Human Anatomy, Sun Yat-sen Medical College of Sun Yat- sen University from May 2003 to December 2004. ①Thirty clean and health SD rats of 18-20 months old, either gender and 400-600 g weight were randomly divided into control group, high dose ginseng group and low dose ginseng group with 10 rats in each group.②High dose ginseng group： Rats were given intragastric infusion of 3 mL ginseng decoction containing 1.5 g/kg per day crude drug （Jilin Panax Ginseng was bought from Guangzhou Medicinal Materials Company. Water decoction was prepared after 2-hour decocting in octuple water and 1.5-bout decoction in sextuple water, then concentrated as containing 2 kg/L ginseng）; Low dose ginseng group： Rats were given intragastric infusion of 3 mL decoction containing 0.5 g/kg per day ginseng while 3 mL distilled water in control group, continuously for three weeks in each group.③Three weeks later, rats were anesthetized to isolate prefrontal cortex, hippocampus, cerebollar cortex, thalamus, hypothalamus, pons and collum of spinal cord. Then, the β-AR contents in above locations were analyzed by radioligand receptor binding assay. ④One-factor analysis of variance was performed initially, then Student-Newman-keals test was applied for statistic data of three groups. RESULTS： Totally 30 rats were involved into the result analysis.①β-AR contents in brains of center cortex structure： After 3-week administration, the β-AR contents in prefrontal cortex, hippocampus and cerebellar cortex were significantly higher in ginseng groups than in control group （t=2.71-4.21, P 〈 0.05-0.01）, especially in high dose ginseng group.②No significant difference was found in β-AR contents of thalamus, hypothalamus, pons and collum of spinal cord of three groups （P 〉 0.05）. CONCLUSION： Ginseng can sdectively increase β-AR contents of prefrontal cortex, hippocampus and cerebellar cortex, especially high dose ginseng decoction （containg 1.5 g/kg per day）. However, ginseng is ineffective for β-AR contents of thalamus, hypothalamus, pons and collum of spinal cord.