紫花龙胆水煎液对气管纤毛运动的影响

Effect of gentianaceous flower decoction on trachea cilia movement

目的:观察紫花龙胆水煎液对大鼠离体气管纤毛运动的影响,以及对10g/L博莱霉素引起纤毛损伤的保护作用。方法:实验于2004-08/2005-02在解放军第四军医大学基础部药理科完成。①选用15只雌性SD大鼠,8周龄。取出喉头至气管分叉处的整段气管。每条气管分成4段,每段气管纵向剪成两半,分别在3个培养皿中倒入龙胆花水煎液[称取干燥龙胆花2.2g,加300mL冷蒸馏水浸泡15min,煮沸30min,过滤药渣加100mL水继续煎煮,煮沸10min,过滤合并两次滤液水浴浓缩成80mL,加NaCl8mg药物浓度27.5g/L(相当于生药2.2g)],10g/L博莱霉素[15mg(效价)日本化药株式会社生产,用时用生理盐水配制成10g/L溶液],生理盐水。每只大鼠可获取4个标本。②将15只大鼠的60个标本随机分为4组:生理盐水组、紫花龙胆组、博莱霉素组和博莱霉素+紫花龙胆花组。用20倍解剖显微镜直接观察气管片,用秒表及两脚规测量墨汁运行速率,每次观察1min。每观察1次后,将气管片漂洗2次,以除去墨汁,5min后将相应离体气管片分别在生理盐水(生理盐水组)、紫花龙胆水煎液(紫花龙胆水煎液组)、10g/L博莱霉素液(博莱霉素组)中浸沾2min按上方法观察不同药物对气管纤毛墨汁运行速率的影响。博莱霉素+紫花龙胆花组:先将标本在10g/L博来霉素溶液中浸沾2min后,漂洗10min,再浸入紫花龙胆水煎液中2min后记录墨汁运行速率。墨汁清除率(%)=(给药后运行速率-给药前运行速率)/给药前运行速率。③组间计量资料差异比较采用方差分析及Dunnett-t检验,组内差异比较采用配对t检验。结果:大鼠15只均进入结果分析。①离体气管纤毛运行速率:紫花龙胆组较用药前明显加快,博莱霉素组较用药前明显减慢。②墨汁清除率:紫花龙胆组较用药前增加了37%,明显高于生理盐水组(P<0.05),博莱霉素组较用药前降低了44%,明显低于生理盐水组(P<0.01),博莱霉素+紫花龙胆组明显高于博莱霉素组(P<0.01)。结论:10g/L博莱霉素液可致气管黏膜纤毛上皮对墨汁清除能力减退,紫花龙胆水煎液则有增强气管纤毛上皮清除墨汁的能力,可显著改善由10g/L博莱霉素溶液所致纤毛上皮运动能力受损,增强排痰功能。

AIM： To observe the effect of gentianaceous flower decoction on the isolated trachea cilia movement of rat, and discover its preventive effect on cilia injury induced by 10 g/L bleomycin. METHODS： This experiment was completed in the Department of Pharmacology, Fourth Military Medical University of Chinese PLA from September 2004 to February 2005. ② Fifteen female SD rats aged 8 weeks were selected. The whole trachea was taken out from larynx to branches of trachea. Each piece of trachea was divided into 4 sections and every sec- tion was cut into half lengthways. Three plates were added with gentianaceous flower decoction （2.2 g dried gentianaceous flower were dipped into 300 mL cold distilled water for 15 minutes and boiled for 30 minutes. After filtration, it was boiled in 100 mL water continuously for 10 minutes. All filtrate was collected and concentrated to 80 mL liquid which was added with 8 mg NaCl. The final concentration was 27.5 g/L which was equal to 2.2 g raw materials）, 10 g/L bleomycin （15 mg, diluted by saline, Nippon Kayaku Co Ltd） and saline. Four samples would be obtained from every rat. ② All 60 samples of 15 rats were randomly divided into 4 groups： saline group, gentianaceous flower decoction group, bleomycin group and bleomycin ＋ gentianaceeus flower decoction group. 20-time amplification anatomy microscope was used to observe the trachea directly, and the speed of ink movement was tested with stopwatch and compasses for 1 minute. The trachea was washed twice to clean the ink, 5 minutes before every observation, the trachea was dip into saline （saline group） gentianaceous flower decoction （gentianaceous flower decoction group）.and 10 g/L bleomycin （bleomycin group） for 2 minutes ＇to Observe effect of various medicines on ink movement speed of trachea cilia. Bleomycin ＋ gentianaceeus flower decoction group： The trachea was. dipped into 10 g/L bleomycin solution for 2 minutes, washed for 10 minutes, then dipped into gentianaceous flower decoction for 2 minutes to record the speed of ink movement. Rate of ink cleaning （%）=（spoed after using drags - speed before using drags）/speed before using drags. ③ Meas. urement data were compared with analysis of variance and Dunnett-t test among groups, and paired t test was used to compare.measurement data inthe same group. RESULTS： AII .15 rats were involved in the final analysis. ① The-speed of trachea cilia in vitro： Spoed.in gentianaceous ttawer decoction group was increased remarkably after administration, but was decreased after administration in bleomycin group. ② Rate of ink denning： Rate in gentianaceous＇ flower decoction group was increased 37% after.administration, which was higher than that in saline group （P 〈 0.05）; Rate in bleomycin group was decreased 44% after administration, which was lower than that in saline group （P 〈 0.01）, and that in bleomyein ＋ gentianaceous flower decoction group was higher than that in bleomycin group （P 〈0.01）. CONCLUSION： 10 g/L bleomycin can restrain trachea cilia movements, and gentianaceous flower decoction can remove sputum by increasing trachea cilia movement and also can protect motor function injury of epithelial cilia.