淫羊藿、鹌鹑复方对免疫力低下小鼠免疫功能的影响

Effect of Yinyanghuo and Anchun compound on immunologic function of mice with hypoimmunity

目的:观察验方淫羊藿、鹌鹑复方对免疫力低下小鼠免疫功能的影响。方法:实验于2005-09/10在三峡大学医学院天然药物实验室完成。①选用昆明系雄性小鼠30只,鼠龄三四个月,体质量(20±2)g。将昆明系雄性小鼠随机分为3组:空白对照组、模型组和中药复方治疗组,每组10只。②空白对照组:实验进程中正常饲养不作任何处理;模型组:腹腔内注射80mg/kg环磷酰胺造成免疫力低下模型,1次/d,同时灌服生理盐水25mL/(kg·d);中药复方治疗组:腹腔内注射80mg/kg环磷酰胺造成免疫力低下模型,1次/d,同时灌胃淫羊藿、鹌鹑复方粉剂溶解液(复方粉末来源于淫羊藿、鹌鹑复方胶囊,由三峡大学第一临床医学院和长阳县人民医院制备,其主要成分为淫羊藿、鹌鹑、肉苁蓉等中药材。淫-鹑胶囊为医院制剂,2g/粒)25g/(kg·d)。3组均连续干预10d。③用药10d后,取其脾制成脾细胞悬液,采用流式细胞仪检测脾组织T细胞亚群。④组间计量结果差异比较采用t检验。结果:30只小鼠均进入结果分析。①模型组小鼠脾脏CD3+,CD4+T细胞亚群百分比和CD4+/CD8+明显低于空白对照组[(36.95±2.17)%,(26.27±2.58)%,1.61±0.21;(47.11±1.72)%,(36.14±1.95)%,1.89±0.12,P<0.05～0.01];中药复方治疗组明显高于模型组[(44.04±2.02)%,(28.20±1.37)%,2.23±0.11,P<0.05～0.01]。②模型组小鼠脾脏CD8+T细胞亚群百分比明显低于空白对照组[(16.34±1.72)%,(19.16±2.02)%,P<0.05],高于中药复方治疗组[(12.65±1.12)%,P<0.01]。结论:淫羊藿、鹌鹑复方能够显著改善免疫力低下小鼠的免疫功能。

AIM： To observe the effect of Yinyanghuo and Anchun compound on the immunologic function of mice with hypoimmunity. METHODS： The experiment was conducted at the Natural Drug Laboratory of the Three Gorges University Medical College from September to October 2005. ①Thirty male Kunming mice aged 3-4 months with body mass of 20±2 g were randomly assigned into three groups： Blank control group, model group and treatment group with ten mice in each group. ② Blank control group： The mice were fed with normal diet without any treatment; the model group： the mice were intraperitoneally injected with 80 mg/kg cyclophosphamide once daily and orally saline 25 mL/kg every day to prepare the hypoimmunity model; the treatment group： the mice were intraperitoneally injected with 80 mg/kg cyclophosphamide once daily to establish the hypoimmunity model, meanwhile, the Yinyanghuo and solution of Anchun compound powder were infused into the stomach of the hypoimmunity mice model, 25 g/kg daily [the compound power was from the Yinyanghuo and Anchun Compound capsule （Yin-Chun capsule）, which was prepared by the First Clinical Medical College of Three Gorges University and the People＇s Hospital of Changyang and composed of Yinyanghuo, A nchun, Roucongrong and other Chinese herbs. The Yin- Chun capsule was hospital preparation, 2 g per pill]. All the three groups were given 10 days intervention. ③10 days later, the spleen was removed to prepare the spleen cell suspension, and the T cell subpopulation of the spleen tissue was examined by the flow cytomcter. ④Comparison of difference of measurement result among groups was performed with t-test. RESULTS： All the 30 mice ＂were involved in the result analysis. ①The percentage of the CD^3＋ T, CD^4＋ T Cell subpopulation and the level of CD^4＋ /CD8^＋ in the model group was obviously lower than the control group and the treatment group [（36,95±2.17）%, （26.27±2.58）%, 1.61±0.21; （47.11 ±1.72）%, （36.14±1.95）%, 1.89±0.12, P 〈 0.05-0.01], [（44.04±2.02）%, （28.20±137）%, 2.23±0.11, P 〈 0.05-0.01], respectively. ②The percentage of CD8^＋ T cell subpopulation in the model group was markedly lower than the blank control group [（16.34±1.72）%0 （19.16±2.02）%, P 〈 0.05], but higher than that in the treatment group [（12.65±1.12）%, P 〈 0.01]. CONCLUSION： The Yinyanghuo and A nchun compound can significantly improve the immunologic function of the mice with hypoimmunity.