摘要
目的:观察验方淫羊藿、鹌鹑复方对免疫力低下小鼠免疫功能的影响。方法:实验于2005-09/10在三峡大学医学院天然药物实验室完成。①选用昆明系雄性小鼠30只,鼠龄三四个月,体质量(20±2)g。将昆明系雄性小鼠随机分为3组:空白对照组、模型组和中药复方治疗组,每组10只。②空白对照组:实验进程中正常饲养不作任何处理;模型组:腹腔内注射80mg/kg环磷酰胺造成免疫力低下模型,1次/d,同时灌服生理盐水25mL/(kg·d);中药复方治疗组:腹腔内注射80mg/kg环磷酰胺造成免疫力低下模型,1次/d,同时灌胃淫羊藿、鹌鹑复方粉剂溶解液(复方粉末来源于淫羊藿、鹌鹑复方胶囊,由三峡大学第一临床医学院和长阳县人民医院制备,其主要成分为淫羊藿、鹌鹑、肉苁蓉等中药材。淫-鹑胶囊为医院制剂,2g/粒)25g/(kg·d)。3组均连续干预10d。③用药10d后,取其脾制成脾细胞悬液,采用流式细胞仪检测脾组织T细胞亚群。④组间计量结果差异比较采用t检验。结果:30只小鼠均进入结果分析。①模型组小鼠脾脏CD3+,CD4+T细胞亚群百分比和CD4+/CD8+明显低于空白对照组[(36.95±2.17)%,(26.27±2.58)%,1.61±0.21;(47.11±1.72)%,(36.14±1.95)%,1.89±0.12,P<0.05~0.01];中药复方治疗组明显高于模型组[(44.04±2.02)%,(28.20±1.37)%,2.23±0.11,P<0.05~0.01]。②模型组小鼠脾脏CD8+T细胞亚群百分比明显低于空白对照组[(16.34±1.72)%,(19.16±2.02)%,P<0.05],高于中药复方治疗组[(12.65±1.12)%,P<0.01]。结论:淫羊藿、鹌鹑复方能够显著改善免疫力低下小鼠的免疫功能。
AIM: To observe the effect of Yinyanghuo and Anchun compound on the immunologic function of mice with hypoimmunity.
METHODS: The experiment was conducted at the Natural Drug Laboratory of the Three Gorges University Medical College from September to October 2005. ①Thirty male Kunming mice aged 3-4 months with body mass of 20±2 g were randomly assigned into three groups: Blank control group, model group and treatment group with ten mice in each group. ② Blank control group: The mice were fed with normal diet without any treatment; the model group: the mice were intraperitoneally injected with 80 mg/kg cyclophosphamide once daily and orally saline 25 mL/kg every day to prepare the hypoimmunity model; the treatment group: the mice were intraperitoneally injected with 80 mg/kg cyclophosphamide once daily to establish the hypoimmunity model, meanwhile, the Yinyanghuo and solution of Anchun compound powder were infused into the stomach of the hypoimmunity mice model, 25 g/kg daily [the compound power was from the Yinyanghuo and Anchun Compound capsule (Yin-Chun capsule), which was prepared by the First Clinical Medical College of Three Gorges University and the People's Hospital of Changyang and composed of Yinyanghuo, A nchun, Roucongrong and other Chinese herbs. The Yin- Chun capsule was hospital preparation, 2 g per pill]. All the three groups were given 10 days intervention. ③10 days later, the spleen was removed to prepare the spleen cell suspension, and the T cell subpopulation of the spleen tissue was examined by the flow cytomcter. ④Comparison of difference of measurement result among groups was performed with t-test.
RESULTS: All the 30 mice "were involved in the result analysis. ①The percentage of the CD^3+ T, CD^4+ T Cell subpopulation and the level of CD^4+ /CD8^+ in the model group was obviously lower than the control group and the treatment group [(36,95±2.17)%, (26.27±2.58)%, 1.61±0.21; (47.11 ±1.72)%, (36.14±1.95)%, 1.89±0.12, P 〈 0.05-0.01], [(44.04±2.02)%, (28.20±137)%, 2.23±0.11, P 〈 0.05-0.01], respectively. ②The percentage of CD8^+ T cell subpopulation in the model group was markedly lower than the blank control group [(16.34±1.72)%0 (19.16±2.02)%, P 〈 0.05], but higher than that in the treatment group [(12.65±1.12)%, P 〈 0.01].
CONCLUSION: The Yinyanghuo and A nchun compound can significantly improve the immunologic function of the mice with hypoimmunity.
出处
《中国临床康复》
CSCD
北大核心
2006年第15期148-150,共3页
Chinese Journal of Clinical Rehabilitation