柴胡黄芩不同配伍比例干预急性酒精性肝损伤大鼠肝脾、胸腺、肾上腺指数及肾上腺中维生素C含量的变化(英文)

Interventional effect of radix bupleuri and radix scutellariae at different ratios on the changes in the indexes of liver, spleen, thymus gland and suprarenal gland and vitamin C in suprarenal gland of rats with acute alcoholic liver injury

背景:现代医学对酒精性肝损伤的发病机制尚未完全清楚,除了戒酒、支持、对症治疗外,尚无特效的治疗方法。中医药对本病的治疗有着明显的优势,既能改善临床症状又能调理机体免疫功能,有着较好的发展前景。目的:从整体观念出发探讨柴胡、黄芩以不同剂量比例配伍(2∶1,1∶1,1∶2)对大鼠急性酒精性肝损伤的防治作用。设计:完全随机分组设计,空白对照实验。单位:河南中医学院药学院临床中药学实验室。材料:实验于2002-09/11在河南中医学院临床中药学实验室完成。选用健康Wistar大鼠96只,雄性。随机将96只大鼠分为8组:空白组,模型组,柴胡组,黄芩组,小柴胡组,柴胡黄芩2∶1组,柴胡黄芩1∶1组,柴胡黄芩1∶2组,每组12只。方法:①按质量比2∶1,1∶1,1∶2分别称取柴胡、黄芩(480g,240g;360g,360g;240g,480g)制备柴胡水煎液。除空白组(以等体积的生理盐水溶液灌胃)外,其余各组均灌胃给予56度二锅头白酒7mL/kg,2次/d,连续10d造模结束。自造模第1天起,每日灌胃给药2次。空白组和模型组灌服等体积的蒸馏水,其余各组分别灌胃给予相应的药物水煎液,小柴胡组剂量为10g/kg,其余各给药组剂量皆为12g/kg。②末次给酒后16h,腹主动脉取血制备血清。迅速摘取肝脏、脾脏、胸腺、肾上腺。计算脏器指数:脏器指数脏器质量(g)/体质量(g)×100%。计算肾上腺维生素C含量(mg/g):狖[(测定管A值-空白管A值)/(标准管A值-空白管A值)]×标准管浓度(6mg/L)×样品测试前的稀释倍数/组织中蛋白含量(g/L)狚。③组间计量资料比较采用方差齐性检验及单因素方差分析。主要观察指标:各组大鼠肝脏、脾脏、胸腺、肾上腺(右侧)指数及右侧肾上腺中维生素C含量比较。结果:实验过程中模型组大鼠死亡3只,柴胡组、柴胡黄芩1∶1组各死亡2只,其余各组均死亡1只,进入结果分析空白组12只,模型组9只,柴胡组、柴胡黄芩1∶1组各10只,其余各组11只。①各组大鼠肝脏、脾脏、胸腺、肾上腺(右侧)指数的比较:各组大鼠肝脏指数差异不明显。模型组脾脏指数、胸腺指数、肾上腺指数均明显低于空白组(P<0.05～0.01)。柴胡组脾脏指数明显高于模型组,能使降低的脾脏指数趋于正常(P<0.05);黄芩组、柴胡黄芩1:1组、1:2组、小柴胡组胸腺指数明显高于模型组(P<0.05);柴胡黄芩1:2组、小柴胡组肾上腺指数明显高于模型组(P<0.05)。②各组大鼠右侧肾上腺中维生素C含量比较:模型组肾上腺组织中维生素C含量与空白组比较,差异不明显。柴胡组、小柴胡组、柴胡黄芩配伍组肾上腺中维生素C含量均明显低于模型组(P<0.05～0.01),以柴胡黄芩1∶1组的作用趋势最显著(P<0.01)。结论:干预肝脾、胸腺、肾上腺功能损伤可能是柴胡黄芩配伍抗急性酒精性肝损伤的机制之一;各配伍比例中以柴胡黄芩1∶2组作用最明显。

BACKGROUND：. Until now modem science cannot explain cleady about the mechanism of acute alcoholic liver injury： Besides such methods as giving up drinking, or symptomatic treatment, there isno specific therapy and remedy. Traditional Chinese Medicine （TCM） has obvious advantages for this： It cannot only improve the clinical symptoms, but also adjust organism immune function, and has very good development future. OBJECTIVE： To study the effects of radix bupleuri and radix scutellariae in compatibility with the ratio of different dosage （2：1, 1：1, 1：2） on acute alcoholic liver injury by regulating the system of eridoerine-immunity network in rats. DESIGN： Groups are divided randomly and experiments are made under blank conditions. SETTING： TCM Clinical Laboratory of Pharmaceutical School, Henan University of TCM. MATERIALS： From September to November 2002＂in TCM Clinical Laboratory of Pharmaceutical School, Henan University of TCM, the general condition of the animals were as follows： 96 healthy Wistar rats were divided into 8 groups： blank group, model group, radix bupleuri group, radix scutellariae group, xiao-ch.aihu group, radix bupleurl-radix seutellariae 2：1 group, radix bupleuri-radix scutellariae 1：1 group, radix bupleuri-radix scutellariac 1：2 group. METHODS： ① According to the ratio of 2：1, hl, 1：2, radix bupleuri and radix scutellariae were taken respectively （480 g, 240 g; 360 g, 360 g;, 240 g, 480 g） to produce the water deeocting medicine. Except the blank group （duplicated by saline of the same volume through consecutive gastric infusion）, the other groups are duplicated through consecutive gastric infusion .by 56 degrees of Er-Guotou White Spirit （7 mL/kg） two times a day for ten days until the model-making is finished. From the first day, the medicine was given two times a day. The blank group and the model group were given the distilled water of the same volume. The other groups were given the proper water deeocting medicine. The dosage in xiao-chaihu group was 10 g/kg and in the other was all !2 g/kg. ②16 hours later after the last time when the white spirit was given, blood was taken from stomach aorta to produce serum. Liver, spleen, chest gland, adrenal gland were quickly removed. The calculating formula of an organ index： An organ index= The weight of an organ （g）/The weight of a body （g） ：〈100%. The calculating forrhula of vi- tamin C content in adrenal gland （mg/g）： Content of vitamin C = [（The A value of the testing tube-The A value of the blank tube）/ （The A value of the standard tube-The A value of the blank tube）] ：〈density of the standard tube （6 mg/L）xdilution times of the sample before tested/protein content （g/L）. ③ Comparison between the groups was shown by using variance similar test and single-factor variance analysis. MAIN OUTCOME MEASURES： Index of rats＇ liver, spleen, chest gland, and adrenal gland （on the right side）; the determination of vitawin C content in rats＇ right adrenal gland. RESULTS： During the experiment, 3 rats in the model group died. Two rats died in radix scutellariae group and radix bupleuri-radix seutellariae group respectively. In each of the other groups only one rat died. The condition of the number of the rats which were used for analyzing results：There were 12 in blank group; 9 in model group; 10 in radix bupleuri group, radix bupleuri-radix scutellariae 1：1 group respectively; 11 in each of the other groups. ① Comparison of index of rats in liver, spleen, iehest gland, adrenal gland （right side） between different groups： There was no significant difference between the indexes of rats＇ liver in different groups. The index of spleen, chest gland, adrenal gland in the model group were all greatly less than in blank group （P 〈 0.05-0.01）. The index of spleen in the radix bupleuri group wcre greatly higher than in the model group, it could make the reduced spleen index return to normal （P〈 0.05）; The index of chest in radix scutellariae group, radix bupleufi-radix scutellariae 1：1 group, radix bupleuri-radix scutellariae 1：2 group and xiao-chaihu group were greatly higher than in model group （P 〈 0.05）; The index of adrenal gland in radix bupleuri-i：adix scutellariae 1：2 group and xiao-chaihu group were greatly higher than in model group （P 〈 0.05）. ②Comparison of vitamin C content in rats＇ right adrenal gland between the groups： Compared with blank group, the vitamin C content of adrenal gland in the model group had no significant difference. While that of radix bupleuri group, xiao-chaihu group, radix bupleuri-radix scutellariae groups were greatly less than that of model group （P 〈 0.05-0.01）, especially radix bupleuri-radix scutellariae 1：1 group had the most significant influence （P 〈 0.01）. CONCLUSION： Interfering functional injuries of liver, spleen and suprarenal gland is one of the mechanisms of acute alcoholic liver injury. Generally speaking, bupleuri-radix scutellariae 1：2 group has the greatest effect on the testing index.