穴位电针刺激帕金森病大鼠纹状体谷氨酸含量的变化(英文)

Changes of content of glutamic acid in striatum of rats with Parkinson disease after electroacupuncture stimulation at acupoints

背景:纹状体内多巴胺和谷氨酸两种神经递质平衡紊乱参与了帕金森病的发病过程。电针在治疗帕金森病的过程中能否调节纹状体谷氨酸含量,从而恢复两种递质的平衡。目的:观察帕金森病大鼠纹状体中兴奋性氨基酸谷氨酸含量的变化以及电针干预对其的影响。设计:随机分组设计、对照实验。单位:湖北中医学院针灸教研室。材料:实验于2002-02/08在湖北中医学院重点学科(针灸)实验室完成。选择清洁级健康SD大鼠40只,随机分成4组,正常组、模型组、假手术组、电针组,每组10只。方法:模型组采用右侧黑质化学损毁法制备帕金森病大鼠旋转模型。假手术组:假手术方法同模型组,注入生理盐水3.4μL。电针组:在造模成功后予以电针治疗。参照《实验动物穴位图谱》,取太冲、风府穴,毫针针刺,接G6805电针治疗仪,采用连续波,频率为2Hz,治疗30min,1次/d,连续治疗7d后取材进行谷氨酸含量测定。主要观察指标:经氨基酸自动分析仪测定正常组、假手术组、模型组、电针组大鼠双侧纹状体谷氨酸含量。结果:模型组大鼠右侧纹状体谷氨酸含量显著高于对侧纹状体及正常组、假手术组、模型组、对侧纹状体、正常组、假手术组分别为(101.6±7.49),(71.4±5.65),(61.8±4.38),(62.6±5.21)μg/g,P<0.01)。而电针组右侧纹状体谷氨酸含量显著低于模型组、电针组、模型组分别为(83.7±5.68),(101.6±7.49)μg/g,P<0.05),但仍高于正常组与假手术组(P<0.05)。各组左侧纹状体谷氨酸含量比较差异无显著性(P>0.05)。结论:兴奋性氨基酸参与了帕金森病的发病过程,减少兴奋性氨基酸的释放是电针治疗帕金森病的作用途径之一。

BACKGROUND： Balance disorder of the two neurotransmitters-dopamine and glutamic acid in stfiatum, participates in the pathogenic process of Parkinson disease. Whether or not electroacupuncutre can regulate the content of glutamic acid in striatum in the treatment of Parkinson disease so as to recover the balance of the two neurotransmitters is needed further investigation. OBJECTIVE： To observe the changes of content of excitatory amino acid （glutamic acid） in stfiatum of rat with Parkinson disease and the effects of electroacupuncture intervention on these changes. DESIGN： Randomized controlled trial. SETTING： Teaching and Research Section of Acupuncture and Moxibustion, Hubei University of Traditional Chinese Medicine. MATERIALS： The experiment was completed in Laboratory for Important Subject （Acupuncture and Moxibustion） of Hubei University of Traditional Chinese Medicine from February to August 2002. A total of 40 healthy SD rats were selected and randomly divided into 4 groups, namely, normal group, model group, sham operation group and eleetroaeupuncutre group with 10 in each group. METHODS： Model group： Rotation model of rat with Parkinson disease was established by method of chemical damage on fight substantia nigra. Sham operation group： Method of sham operation was the same as model group, and 3.4 μL physiological saline was injected. Electroacupuncture group： Electroacupuncture treatment was given after model establishment; took Atlas of Experimental Animd Acupoints as reference, selected the acupoints of Talchong and Fengfu, pierced with needles which were connected with G6805 electroacupuncture therapeutic apparatus under the condition of continuous waves and frequency of 2 Hz for 30 minutes, once a day for consecutive 7 days, then detected the content of glutamic acid. MAIN OUTCOME MEASURES： Content of glutamic acid in bilateral striatum of rat in normal group, sham operation group, model group and electrnacupuncture group, which was detected by automatic amino acid analyzer. RESULTS： The content of glutamic acid in right striatum of rat in model group was significantly higher than that in contralateral striatum, normal group and sham operation group [the contents of glutamic acid in model group, contralateral strlatum, normal group and sbam operation group were （101.6±7.49）, （71.4±5.65）, （61.8±4.38）, （62.6±5.21） μg/g respectively, P 〈 0.01]. Tbe content of glutamic acid in right striatum in electroacupuncture group was significantly lower than that in model group [the contents of glutamlc acid in electroacupuncture group and model group were （83.7±5.68）, （101.6±7.49） μg/g respectively, P 〈 0.05], but still higher than that in normal group and sham operation group （P 〈 0.05）. There was no significant difference between the contents of glutamic acid in left stfiatum in each group （P 〉 0.05）. CONCLUSION： Excitatory amino acid participates in the pathogenic process of Parkinson disease. Therefore, one of the approaches of electroacupuncture treatment for Parkinsonism is reducing the release of excitatory amino acid.