水稻空间诱变稻瘟病抗性变异研究及抗性变异基因的分子标记

Study on resistance variation of space-induced lines to rice blast and molecular marker of the resistant mutant gene

对空间诱变的丽江新团黑谷SP1～SP2代株系稻瘟病抗性变异进行了分析。结果表明，经过空间诱变后。510株SP1代植株中有70株对接种的GD531菌株由感病变为抗病。占总株数的13．7％；SP2代多数抗病株系对接种菌株的抗感分离比例符合3：1或15：1的规律，说明SP2代多数抗病株系受1对或2对显性主效基因控制；SP2代受1对抗病主效基因控制的抗病株系抗性继续分离，而受2对抗病主效基因控制的抗病株系抗性基本稳定；利用LR8-SP2群体对丽江新团黑谷经过空间诱变后产生的抗病基因进行分子标记，结果发现该基因位于第9染色体上，与微卫星标记RM409连锁。

The research data on the variation of the resistance to rice blast in the SP1--SP3 of lijiangxintuanheigu which was susceptible to blast after space-induction showed that 13.7% mutant plants in the SP1 were resistant to rice blast. In the SP2 rice space-induced lines ,most of the resistant lines had a ratio of 3 ： 1 or 15 ： 1 of the deviation of resistance and susceptibility,implying the resistance was controlled by 1 pair or 2 pairs of major genes. In the SP3 rice space-induced lines,a part of lines whose.resistance were controlled by a pair of major genes continued to demonstrate deviation of resistance,but for the lines whose resistance were controlled by 2 pairs of major genes ,the resistance generally became stable. By using the LR8- SP2 population for the molecular markers of the blast resistant genes induced through space mutation,it was found that the resistant gene was in the 9th chromosome and was linked with the SSR marker RM409.