摘要
为提高尿素包合法富集花生四烯酸的效率,通过正交设计对甲醇用量、结晶温度、尿/酯比、降温速度等因素进行分析,发现降温速度对分离效果有显著影响、结晶过程是制约分离效果的瓶颈。测定了油酸、亚油酸的尿素包合物的溶解度和超溶解度。当结晶温度为-7℃、降温速度5℃.h-1、脂肪酸甲酯∶尿素∶甲醇为1 g∶2 g∶12 mL时,花生四烯酸的质量分数为87.02%、收率为88.66%。
In the process of purification of arachidonic acid（AA） from fungi lipids by urea inclusion,orthogonal design were used. Crystallization temperature and speed of cooling-down had an outstanding effect on AA yield. So a crystallography study was carried out. Solubility and super-solubility curve were presented. When the temperature of crystallization was 7℃,the speed of cooling down was 5℃·h^-1 ,ratio of urea/fatty acid/ methanol was 1 g ： 2 g ： 13 mL, AA was separated with a purity of 87.02% （mass fraction）and recovery of 88.66%.
出处
《化学与生物工程》
CAS
2006年第4期29-31,共3页
Chemistry & Bioengineering
基金
江苏省博士后基金资助项目
江苏省自然科学基金资助项目(BK2005114)
关键词
花生四烯酸
尿素包合
富集
降温速度
结晶
arachidonic acid
urea inclusion
purification
speed of cooling-down
crystallization
