大蒜素对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究

Protective Effects of Arlicin on Focal Cerebral Ischemia-reperfusion Injury in Rats

目的研究大蒜素(arlic in)对脑缺血再灌注损伤的保护作用及其可能机制。方法采用线栓法制备大鼠右侧大脑中动脉栓塞缺血再灌注模型,于脑缺血2 h再灌注24 h时,观察不同剂量大蒜素对脑缺血再灌注大鼠的神经功能状态、脑梗死体积、脑含水量、及脑组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-1β(interleuk in-1β,IL-1β)含量的影响。结果大蒜素可明显改善大鼠神经病学症状,降低脑水肿程度,降低脑梗死体积;降低脑组织TNF-α、IL-1β的含量。结论大蒜素对脑缺血再灌注神经元损伤具有良好保护作用,其机制可能与其抗炎作用有关。

Objective To study the protective effects of arlicin on cerebral ischemia -reperfusion injury in rats and explore the mechanisms. Metheds Focal cerebral ischemia - reperfusion model was established in rats through occluding the middle cerebral artery（MCAO） on one sise. The protective effects of arlicin at different doses was evaluated by investigating neurological score, infract size of brain,cerebral water contents,the contents of tumor necrosis factor -α（TNF - α） and interleukin -1β（ IL -1β）. Results Arlicin not only obviously alleviated the neurological symptoms, but also reduced the infract size and water contents. Arlicin decreased the con- tents of TNF -α and IL -1β in brain tissue. Conclusion Arlicin showed obvious protective effects on focal cerebral ischemia -reperfusion injury in rat and the protective effects might work through the anti-inflammation activity of arlicin.