厌氧菌降解四氯乙烯的研究

Study on Tetrachloroethylene Degradation by Anaerobic Bacteria

报道了沼气污泥富集培养物中厌氧菌降解四氯乙烯的特征.研究结果表明.沼气污泥在甲醇、甲酸盐、乙酸盐、乙醇和H_2/CO_2等不同碳源基质中的富集培养物,以利用甲醇为碳源的富集培养物降解四氯乙烯(Tetra-chloroe—thylene,简称PCE,下同)活性最高,平均每升培养液每天降解PCE1400nmol.经对该富集培养物的分离培养,已分离出4株厌氧细菌,其中一株为甲烷八叠球菌,两株为弧菌,一株为杆菌.经对它们进行降解PCE的试验,表明将它们混合回接培养与以上富集培养物有类似降解四氯乙烯的活性.

The characterization of tetrachloroethylene degradation by anaerobic bacteria in Methanogenic sewage sludge-methanol-enrichment culture was discussed.The methanaogenic enrichment culture was formed by adding H2/CC2, acetate, methanol, ethanol as carbon and energy sources. The methanogenic sewage sludge-methanol-enrichment culture had the highest activity, as 1400 n mol/L of the tetrachloroethylene degradation per day.From the methanol-sewage sludge-enrichment culture one methanosarcina, two vibrios and one bacteroid have been isolated. The mixed culture with those isolated species has more high activity for the tetrachloroethylene degradation in the sewage sludge-methanol-enrichment culture.