低剂量辐射对小鼠睾丸生精细胞中细胞色素c和caspase-3表达的影响

Effect of low dose radiation on cytochrome c and caspase-3 protein expressions in spermatogenic cells of mouse testis

目的研究低剂量电离辐射对小鼠睾丸生精细胞细胞色素c(Cytc)和caspase3蛋白表达的影响。方法采用免疫组化法(SABC法),观察低剂量X射线照射后,小鼠睾丸各类生精细胞Cytc和caspase3表达的剂量与时程效应关系。结果0、0.025、0.05、0.075、0.1和0.2Gy照射后12h,小鼠睾丸各类生精细胞不同程度表达Cytc和caspase3,以精原细胞和精母细胞表达为主,精子细胞和精子则表达相对较少,而且,随着剂量的增加,表达也增多。0.075Gy照射后,两种蛋白的表达开始随时间推移而增强,12h达到峰值,然后下降,但仍高于正常组。结论低剂量电离辐射诱导小鼠睾丸生精细胞Cytc和caspase3蛋白的表达具有一定的剂量和时程效应规律性。

Objective To investigate the effect of low dose radiation on the expressions of cytochmme c （ Cyt c） and caspase-3 proteins in spermatogenic cells of mouse testis. Methods The relationships of dose- and time-effect of Cyt c and caspase-3 protein expressions with different dose of X-rays were observed in the spermatogenic cells of mouse testis with immunohistochemieal technique （SABC）, Results After irradiation with 0, 0.025, 0.05, 0.075, 0.1 and 0.2 Gy, Cyt c and caspase-3 proteins expressed differently in all kinds of spermatogenic ceils, and principally in spermatogonia and spermatocytes, and less in spermatids and spermazoa. And the expressions increased with the increasing of irradiation dose. The expressions of both proteins after irradiation with 0.075 Gy increased with the lapse of time and reached to the peak at 12 h, and then descreased. Conclusion Dose-and time-effect exists on the low-dose irradiation induced expressions of Cyt c and caspase-3 proteins in spermatogenie ceils of mouse testis.