姜黄素抗肺纤维化大鼠细胞外基质过度形成的实验研究

Experimental study on protective effects of curcumin on exaggerated extracellular matrix accumulation of pulmonary fibrosis rats

目的:研究姜黄素抗博莱霉素诱导大鼠肺纤维化细胞外基质过度沉积的作用。方法:SD雄性大鼠144只,分为假手术组,模型组,姜黄素高、中、低剂量组,醋酸泼尼松组,每组24只。除假手术组外其余各组大鼠气管内一次性滴注盐酸博莱霉素,假手术组大鼠气管内一次性滴注等体积的生理盐水。造模后第1天开始给药,持续到处死动物的前一天。姜黄素高,中,低剂量组分别为200,100,50 mg.kg-1.d-1,醋酸泼尼松组为0.56 mg.kg-1.d-1,假手术组,模型组分别灌服等体积的生理盐水。各组动物于7,14,28 d随机处死8只,分别观察大鼠肺组织羟脯氨酸(HYP)和血清中Ⅲ型胶原,Ⅳ型胶原及层黏连蛋白和透明质酸的含量,同时取肺组织进行HE,Mallory染色。结果:姜黄素可降低肺纤维化大鼠血清Ⅲ,Ⅳ胶原,层黏连蛋白及透明质酸含量,减轻肺组织纤维性增生。结论:可能是通过降低肺纤维化大鼠细胞外基质含量而发挥治疗肺纤维化作用。

Objective： To study the protective effects of curcumin on exaggerated extracellular matrix accumulation of pulmonary fibro- sis rats. Method： One hundred and forty-four male Sprague-Dawley rats were randomLy divided into 6 groups（24 rats in each group）. Rats in the model control group, positive medicine group, and high, moderate and low cureumin groups were injected with a single dose of bleomycin by trachea, and rats in sham-model control group with same volume normal saline. One day after the injection, cureumin solution of different dosages （200,100,50 mg·kg^-1·d^-1） was respectively given to rats in the high, moderate and low cureumin group daily by gastrogavage, while equal volume of normal saline was given to those in the sham-model control group and model control group, and an equal volume of prednisone（0.56 mg·kg^-1·d^-1） was given to those in positive medicine control group. On the 7, 14, 28 days, 8 rats per treatment group were randomly killed, the levels of Ⅲ -collagen, Ⅳ- collagen, laminin and hyaluronic acid in the serum were determined, the determination of hydroxypmline in lung homogenates was analyzed, and the lung was incised to make pathological sections which were stained with HE and Mallory. Result： Cureumin could decreas the levels of Ⅲ-collagen, Ⅳ-collagen, laminin and hyaluronic acid in the serum,and inhihit the proliferation of fibrous tissue. Conclusion： Curcumin may play its therapetuic role by leveling down the content of extracellular matrix in rats with pulmonary fibrosis induced by bleomycin.