人参配伍五灵脂对A549人肺腺癌细胞增殖抑制及诱导凋亡的实验研究

Experimental study of the effects on proliferation and apoptosis of A549 cell line of adenocarcinoma of the lung with compatibility of Radix ex Rhizoma Ginseng and Fafces Trogopterori

目的:观察人参配伍五灵脂对A549人肺腺癌细胞增殖及凋亡的影响,以阐明其作用机制,探究其最佳配伍比例,为临床用药提供合理的实验依据。方法:健康Wistar大鼠25只,随机分成5组,每组5只,即正常对照组,人参、五灵脂1∶1,1∶2,2∶1组,复方斑蝥胶囊组。煎取药液后,计算出大鼠等效剂量,按1 mL.100 g-1给大鼠灌胃,每天2次,连续灌胃3 d。末次灌胃后1 h取血清,加入RPMI 1640培养液,配制成含量为10%的药物血清温育液。运用MTT法检测肿瘤细胞生长曲线及肿瘤细胞抑制率,透射电镜观察其凋亡情况。结果:人参、五灵脂配伍能抑制A549人肺腺癌细胞增殖,并对其细胞的凋亡有诱导作用,且当二者比例为2∶1时效果最显著。结论:提示抑制肿瘤细胞增殖,诱导其调亡可能是人参配伍五灵脂抗癌作用的机制之一。

Objective： To observe the effects of the compatibility of Radix ex Rhizoma Ginseng and Fafces Trogopterori on proliferation and apoptosis of A549 cell line of adenocarcinoma of the lung, to clarify the mechanism, to explore the best proportion compatibility, and to offer the reasonable experiment evidence in clinical medicine therapy. Method： Twenty-five healthy Wistar rats were divided into five groups randomly, 5 mrs in each group, including normal group, Radix ex Rhizoma Ginseng and Fafces Trogopterori in the ratio of 1 ： 1 group, 1 ： 2 group, 2：1 group, and complex recipe of beetle capstde group. After the pharmacy liquor was decocted, equivalent dose for rat was calculated. According to the weights, all rats were intragastric administrated at the standard of 1 mL·100 g^-1, twice a day, continuously for 3 days. One hour after the last administration, the serum was eolleefed and mixed ~ culture media RPMI 1640 to prepare the drug serum incubation liquid at the concentration of 10%. MTI＇ was used to measure the growth curve and the inhibition rate of tumor cell, and the apoptosis was observed by electron microscope. Result： The eompatibility of Radix ex Rhizoma Ginseng and Fafces Trogopterori could inhibit the cell proliferation of cell line A549 of lung adenocarcinotrm and have an inducement on apoptosis. The effect was significant in the ratio of 2 ： 1. Conclusion：These results indicate that inhibiting the proliferation and inducing the apoptosis of tumor cell may be one of the anficancer mechanism of the compatibility of Radix ex Rhizoma Ginseng and Fafces Trogopterori.