中国六个白灵菇(Pleurotus nebrodensis)商业菌株的DNA指纹图谱分析

DNA Fingerprinting of Six Pleurotus nebrodensis Strains Cultivated Commercially in China

在中国的6个白灵菇商业菌株中,ITS区域的621bpDNA片段和蛋白转录延长因子的519bpDNA片段的序列完全相同,表明该6个菌株属同一个种。RAPD分析结果表明,其中5个白灵菇菌株的亲缘关系较近,用PAUP4.0软件中的UPGMA方法进行聚类分析,遗传差异小于3.4%,但这5个菌株与6号菌株的差异较大,达到了16.9%。在此基础上构建了6号菌株的特异性标记引物。

The distribution of a 621 bp sequence of the ITS region of nuclear rDNA, and a 519 bp fragment of the protein-coding translation elongation factor gene （EF-1α）, among six commercial strains of Pleurotus nebroclensis collected from the main cultivation area of China confirmed that the six strains belonged to the same species. RAPD data indicated that five strains were very closely related, with character differences rated at or below 3.4% using the UPGMA method of PAUP 4.0. Character differences between P. nebrodensis strain No. 6 and the five other strains were rated at 16.9%, and a specific primer for this strain has been identified.