安博维对兔心肌梗死后心功能及基质金属蛋白酶-3表达的影响

Effect of Aprovel Therapy to Heart Function and MMP-3 Expression after Acute Myocardial Infarction in Rabbits

目的:研究兔心肌梗死后基质金属蛋白酶3(MMP-3)在心室重构和心衰形成中的变化及安博维对其的影响。方法:采用兔冠状动脉前降支结扎法建立兔心肌梗死模型。将28只新西兰兔随机分为伪手术组,心肌梗死组,药物干预组。手术前及手术后10周心超检测室间隔厚度(LVSd)、左室后壁厚度(LVPW d),左室射血分数(EF)。用W estern B lot法测定10周后心肌组织的基质金属蛋白酶3的含量。结果:手术后10周时,药物干预组LVSd,LVPW d明显低于心肌梗死组(P<0.05);左室射血分数明显高于心肌梗死组(P<0.05),但低于伪手术组(P<0.05)。心肌梗死后MMP-3表达升高,相对含量与EF呈负相关。结论:MMP-3参与了左室重构和心肌梗死后心衰的形成。安博维通过抑制MMP-3的表达,明显减轻心肌梗死后心肌肥厚,改善左室功能。

Objective： To investigate the change of matrix metalloproteinases during the ventricular remodeling and heart failure induced after acute myocardial infarction in rabbits and the effect of Aprovel.Methods： Rabbits myocardial infarction model was established by permanent ligation of left anterior descending coronary artery. Twenty-eight New Zealand rabbits were randomized divided into 3 groups：sham group, infarcted group and drug treatment group. Pre-operation and 10 weeks after operation, the interventricular septum thickenss （IVSd）, left ventricular posterior wall thickness （LVPWd）, left ventricular ejection fraction（EF） were measured by echocardiography. Expression of MMP-3 protein in myocardium tissues was quantified by Western Blotting. Results： At lOth week, both IVSd and LVPWd had a significant decrease in drug treatment group compared with those of infarcted group （ P 〈 0. 05 ）. EF was obviously increased （ P 〈 0. 05） ,but still lower than sham group（P 〈0. 05）. Expression of MMP-3 protein increased after myocardial infarction. The level of MMP-3 was correlated negatively with the EF. Conclusion： The alteration of MMP-3 contribute to the ventricular remodeling and heart failure induced after acute myocardial infarction.Aprovel can reduce myocardial hypertrophy,improve heart function by reducing the activity of MMP-3.