抵抗素蛋白在妊娠糖尿病孕妇与正常孕妇胎盘的表达

Resistin protein is expression in placental in gestational diabetes mellitus pregnant women and normal pregnant women.

目的了解抵抗素蛋白在妊娠糖尿病孕妇与正常孕妇胎盘的表达情况,探讨抵抗素与妊娠生理性胰岛素抵抗(insu lin resistance,IR)及妊娠糖尿病(gestational d iabetes m ellitus,GDM)的关系。方法W estern b lot法检测妊娠糖尿病孕妇与正常孕妇胎盘抵抗素蛋白表达情况;葡萄糖氧化酶法测定血糖,测量收缩压(SBP)及舒张压(DBP)。结果W estern b lot结果以OD值表示,GDM孕妇胎盘抵抗素蛋白的表达(79305±7645.12)显著高于正常孕妇胎盘的表达(50718±3818.20)(P<0.01)。结论妊娠糖尿病孕妇胎盘抵抗素蛋白的表达显著高于正常孕妇胎盘的抵抗素蛋白的表达;表明胎盘可分泌抵抗素;抵抗素是引起妊娠生理性胰岛素抵抗和妊娠糖尿病的一个细胞因子。

Objective： To explore the expression of resistin protein in placental in gestational diabetes meUitus pregnant women and normal pregnant women and the relationship between pregnant physiological insulin resistane and gestational diabetes meUitus. Methods ： The expression of resistin protein in placental in gestational diabetes meUitus pregnant women and normal pregnant women was measured using western blot method. Fasting serum glucouse concentration was measured by glucouse oxidaseassay. Systolic blood pressure （SBP） and diastolic blood pressure （DBP） were measured. Results： Resistin protein expression in GDM pregnant women placental tissue （79305 ±7645. 12） （arbitarary OD units） was much higher than in placental tissue in normal pregnant women. Conclusion： Resistin protein expression in GDM pregnant women placental tissue is prominent in normal pregnant women placentaL Resistin protein is secreted from human placental tissue. Resistin is a adipocytokine which leads to pregnant physiological insulin resistance and GDM.